Scd14 elisa

Manufactured by R&D Systems

Sourced in United States

The SCD14 ELISA is an enzyme-linked immunosorbent assay designed to quantitatively measure the levels of soluble CD14 (sCD14) in biological samples. sCD14 is a protein that plays a role in the immune response.

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Lab products found in correlation

Stnfr 1 and 2, by R&D Systems (1 mentions)

Close spelling variants of this product

Human sCD14 DuoSet ELISA ELISA kit for Human sCD14 Human sCD14 Quantikine ELISA kit Human sCD14 ELISA kit Human sCD14 Quantikine ELISA SCD14 ELISAs

3 protocols using scd14 elisa

Vitamin D, Bone, and Inflammatory Biomarkers

Cited 2 times

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Screening laboratories were performed at local CLIA certified laboratories. Serum samples were stored at −70°C until batched analysis at the Irving Institute Biomarkers Core at Columbia University Medical Center (New York, NY). We measured 25(OH) D₂ and D₃ by liquid chromatography tandem mass spectrometry; intact PTH (radioimmunoassay; Scantibodies, Santee, CA); N-terminal propeptide of procollagen type 1 (P1NP; RIA; IDS, Scottsdale, AZ); C-telopeptide (CTX; ELISA; IDS Scottsdale, AZ); IL-6 (ELISA; R&D Systems, Minneapolis, MN); soluble receptors of TNFα (sTNFr-I and –II; ELISA; R&D Systems, Minneapolis, MN), and soluble CD14 (sCD14, ELISA; R&D Systems, Minneapolis, MN). Inflammatory biomarkers were chosen based upon association with relevant endpoints (43 (link)-46 (link)). Except for 25(OH)D, biomarkers were measured in duplicate and values averaged for analysis.

Overton E.T., Chan E.S., Brown T.T., Tebas P., McComsey G.A., Melbourne K.M., Napoli A., Hardin W.R., Ribaudo H.J, & Yin M.T. (2015). High-Dose Vitamin D and Calcium Attenuates Bone Loss with Antiretroviral Therapy Initiation: A Prospective, Randomized Placebo-Controlled Trial for Bone Health in HIV-Infected Individuals. Annals of internal medicine, 162(12), 815-824.

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Quantifying Serum sCD14 via ELISA

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Serum samples were collected after centrifugation, and quantified using sCD14 ELISA (R&D systems, Minneapolis, MN) kit in accordance with the manufacturer’s instructions.

Avila D.V., Myers S.A., Zhang J., Kharebava G., McClain C.J., Kim H.Y., Whittemore S.R., Gobejishvili L, & Barve S. (2017). Phosphodiesterase 4b expression plays a major role in alcohol-induced neuro-inflammation. Neuropharmacology, 125, 376-385.

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Plasma sCD14 Levels in LPS-Treated Monkeys

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To verify that the Ch-RMs treated with LPS generated an effective response, we tested sequential plasma samples from all treated monkeys. Plasma sCD14 levels were measured using a commercially available sCD14 ELISA (R&D Systems, USA). Plasma was diluted to 1/200 and assays were performed in duplicate according to the manufacturer's protocol.

Zhang G.H., Wu R.D., Zheng H.Y., Zhang X.L., Zhang M.X., Tian R.R., Liu G.M., Pang W, & Zheng Y.T. (2015). Lipopolysaccharide Increases Immune Activation and Alters T Cell Homeostasis in SHIVB'WHU Chronically Infected Chinese Rhesus Macaque. Journal of Immunology Research, 2015, 202738.

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