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Anti eif2alpha

Manufactured by Cell Signaling Technology
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Anti-eIF2alpha is a primary antibody that specifically recognizes the alpha subunit of the eukaryotic translation initiation factor 2 (eIF2). eIF2 is a protein complex that plays a crucial role in the initiation of protein synthesis by facilitating the binding of the initiator tRNA to the ribosome.

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Lab products found in correlation

Anti phospho eif2 alpha ser51, by Cell Signaling Technology (2 mentions) Anti pkr, by Santa Cruz Biotechnology (1 mentions) Anti irs 1, by Santa Cruz Biotechnology (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions) Anti jnk, by Santa Cruz Biotechnology (1 mentions) Anti pirs1, by Merck Group (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti p70s6k, by Cell Signaling Technology (1 mentions) Chloroquine c6628, by Merck Group (1 mentions) Anti bip, by Cell Signaling Technology (1 mentions) Anti phospho p70s6k thr389, by Cell Signaling Technology (1 mentions) Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions) Anti ulk1, by Cell Signaling Technology (1 mentions) Anti lc3a b, by Cell Signaling Technology (1 mentions) Anti phospho perk thr980, by Cell Signaling Technology (1 mentions) Anti tuberin tsc2, by Cell Signaling Technology (1 mentions) Anti mouse na931, by GE Healthcare (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions)

4 protocols using anti eif2alpha

1

Comprehensive Immunoblotting Antibody Panel

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Anti-PKR (sc-708, Santa Cruz), anti-peIF2alpha (44728G, Invitrogen), anti-eIF2alpha (#9772, Cell Signaling), anti-pJNK (#4668, Cell Signaling), anti-JNK (sc-7345, Santa Cruz), anti-pIRS1 (07–247, Millipore), anti-IRS1 (sc-559, Santa Cruz), anti-pIKKbeta (#2694, Cell Signaling), anti-IKKbeta (#2370, Cell Signaling), and anti-Tubulin (T9026, Sigma).
Taga M., Mouton-Liger F., Sadoune M., Gourmaud S., Norman J., Tible M., Thomasseau S., Paquet C., Nicoll J.A., Boche D, & Hugon J. (2018). PKR modulates abnormal brain signaling in experimental obesity. PLoS ONE, 13(5), e0196983.
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2

Cellular Protein Expression Analysis

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The following primary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA): anti-BiP (#3177), anti-LC3A/B (#4108), anti-Phospho-p70S6K (Thr389) (#9205), anti-p70S6K (#9202), anti-Phospho-ULK1 (Ser757) (#14202), anti-ULK1 (#6439), anti-phospho-PERK (Thr980) (#3179), anti-PERK (#3192), anti-Phospho-eIF2-alpha (Ser51) (#9721), anti-eIF2-alpha (#9722) anti-Tuberin/TSC2 (#4308). Anti-β-actin and anti-α-tubulin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-p62 (GP62-C-WBC) was obtained from ProGen (Heidelberg, Germany). Anti-Lamin A/C/B1 (EPR4068) was obtained from Abcam. Anti-prelamin A (#MABT345) was obtained from Millipore. Anti-p27 Kip1/CDKN1B (sc-1641) was obtained from Santa Cruz Biotechnology. The following secondary antibodies HRP-conjugated were used: anti-Rabbit (NA934) and anti-Mouse (NA931) were obtained from GE Lifesciences (Marlborough, MA, USA). Anti-Guinea pig HRP-conjugated (90001) was purchased by ProGen (Heidelberg, Germany). Chloroquine (C6628) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
González-Blanco C., Marqués P., Burillo J., Jiménez B., García G., Benito M, & Guillén C. (2022). Cell immortalization facilitates prelamin A clearance by increasing both cell proliferation and autophagic flux. Aging (Albany NY), 14(5), 2047-2061.
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3

Western Blot Antibody Validation Protocol

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To evaluate protein expression on Western blot membranes, the following antibody were used: rabbit polyclonal anti-ATF6 (1:600) (Proteintech, 24169-1-AP), rabbit polyclonal anti-XBP1 (1:1000) (Novus Biologicals, NB100-80861), rabbit polyclonal anti-phospho-eIF2alpha (Ser51) (1:1000) (Cell Signaling, 9721), rabbit polyclonal anti-eIF2alpha (1:4000) (Cell Signaling, 9722), rabbit polyclonal anti-BiP/GRIP78 (1:5000) (Proteintech, 11587-1-AP), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, 15204-1-AP), mouse monoclonal anti-DUSP5 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA sc-393801), mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc., sc-7383), rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc., sc-93), rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc., sc-154), rabbit monoclonal anti-Mcl1 (1:1000) (Cell Signaling, 39224), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., sc-126). Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich) was used as the loading control. The goat antimouse IgG-HRP (1:30,000) (Bethyl Laboratories, A90-116P) and goat antirabbit IgG-HRP (1:30,000) (Bethyl Laboratories, A120-101P) were used as secondary antibodies. All primary and secondary antibodies were diluted in PBS–0.1% Tween20 solution containing 3% of BSA (SERVA).
Benedetti R., Gilardini Montani M.S., Romeo M.A., Arena A., Santarelli R., D’Orazi G, & Cirone M. (2021). Role of UPR Sensor Activation in Cell Death–Survival Decision of Colon Cancer Cells Stressed by DPE Treatment. Biomedicines, 9(9), 1262.
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4

Immunochemistry Antibody Characterization

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Sources of commercial antibodies were as follows: anti-FUS (Santa Cruz, Dallas, TX, USA; 4H11), anti-Pur-alpha (Abcam, Cambridge, UK; ab77734), anti-Pur-alpha (Abcam; ab79936), anti-HA (Santa Cruz; Y-11), anti-HA (Santa Cruz; F-7), anti-Flag (Sigma-Aldrich, St. Louis, MO, USA; M2 and M2 affinity gel), anti-Flag fluorescein isothiocyanate (FITC) conjugated (Sigma-Aldrich; M2), anti-Phospho-eIF2-alpha (Cell Signaling Technology, Beverly, MA, USA; D9G8), anti-eIF2-alpha (Cell Signaling Technology; D7D3), anti-cleaved caspase-3 (Cell Signaling Technology; Asp175), anti-NeuN (Merck Millipore, Billerica, MA, USA; A60), anti-TIAR (BD Biosciences, Erembodegem, Belgium), anti-GAPDH (Chemicon-Merck Millipore), anti-puromycin 3RH11 monoclonal antibody (Kerafast, Boston, MA, USA), anti-FUS (Abcam; ab84078), anti-Islet-1/2 (DSHB, Iowa City, IA, USA; 39.4D5). Mouse monoclonal antibody specific for ribosomal protein S19 were prepared in our laboratory.31 (link) FITC, Rhodamine, and aminomethylcoumarin-conjugated affinity-purified secondary anti'bodies, selected for absent cross-reaction, were from Jackson ImmunoResearch (West Grove, PA, USA).
Di Salvio M., Piccinni V., Gerbino V., Mantoni F., Camerini S., Lenzi J., Rosa A., Chellini L., Loreni F., Carrì M.T., Bozzoni I., Cozzolino M, & Cestra G. (2015). Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death & Disease, 6(10), e1943-.
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