Ysi 2900d

Concentrations of glucose (c_Glc), glutamine (c_Gln), and lactate (c_Lac) were measured with the YSI 2900D (Yellow Springs Instruments) biochemistry analyzer. The concentration of ammonia (c_Amm) was determined with an enzymatic test kit (AK00091; NZYTech, Portugal).

Concentrations of glucose (cGlc), glutamine (cGln), and lactate (cLac) were quantified with the YSI 2900D (Yellow Springs Instruments, USA) biochemistry analyzer. The ammonium concentration (cAmm) was determined with an enzymatic test kit (AK00091, nzytech, Portugal). The antibody titer (cAb) was determined using bio‐layer interferometry (Octet RED, Pall ForteBio, USA) with protein A biosensors (Pall ForteBio). Amino acid concentrations were determined from pre‐column derivatized supernatant (ortho‐phthalaldehyde method) by reversed‐phase chromatography using a Poroshell HPH‐C18 column (125 x 4.6 mm, pore size 0.5μm, Agilent Technologies, USA) with fluorometric detection [38].

The cell concentration was measured with the particle counter Z2 (Beckman-Coulter). Viability was determined with the DAPI (4 ${}^{\prime }$ ,6-diamidino-2-phenylindole) method, as explained in [16 (link)]. Concentrations of glucose (c_Glc), glutamine (c_Gln), and lactate (c_Lac) were quantified with the YSI 2900D (Yellow Springs Instruments, USA) biochemistry analyzer and ammonium (c_Amm) with an enzymatic test kit (AK00091, nzytech, Portugal). The antibody titer (c_Ab) was determined with a bio-layer interferometry (Octet RED; Pall ForteBio, USA) with protein A biosensors (Pall ForteBio), according according to the manufacturer’s protocol. In case study I, the concentrations after the cell transfer were calculated.