Pyolysin protein (628,338 HU/mg protein) was generated and purified from the plo plasmid pGS5910 (link),13 (link). Streptolysin O was stored as 1 mg/ml solution and activated with 10 mM dithiothreitol, and Staphylococcus aureus α-hemolysin was stored as 0.5 mg/ml solution according to the manufacturer’s instructions (all Merck).
Staphylococcus aureus α hemolysin
Manufactured by Merck Group
Staphylococcus aureus α-hemolysin is a protein secreted by the bacterium Staphylococcus aureus. It functions as a pore-forming toxin that can disrupt cell membranes.
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Lab products found in correlation
2 protocols using staphylococcus aureus α hemolysin
1
Pyolysin Protein Purification and Activation
2
Purification and Activation of Pore-Forming Toxins
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The plo plasmid (pGS59) was a gift from Dr H Jost (University of Arizona), and the pyolysin protein was generated and purified as described previously, with a specific activity of 628,338 hemolytic units (HU)/mg protein (21 (link), 30 (link)). Streptolysin O was purchased, stored as a 1 mg/ml solution, and activated with 10 mM dithiothreitol according to the manufacturer’s instructions (Merck). Staphylococcus aureus α-hemolysin was purchased and stored as a 0.5 mg/ml solution, according to the manufacturer’s instructions (Merck).
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