Ftc 133

The human normal thyroid cell Nthy-ori 3-1was purchased from Jennio biotech (Guangzhou, China). DTC cell line FTC-133 and human retinal microvascular endothelial cells (RCECs) were obtained from Procell life science and technology (Wuhan, China). DTC cell line B-CPAP, human BHT-101 cells and ACT cells were obtained from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The Nthy-ori 3 − 1, BHT101, B-CPAP, BHT-101 and ACT cell line were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma, St. Louis, MO, USA), 100 U/ml of penicillin G and 100 μg/ml of streptomycin. RCECs were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco Life Technologies, Rockville, MD, USA) supplemented with 10% FBS, 100 U/ml of penicillin G and 100 μg/ml of streptomycin. The cultures were maintained in a humidified atmosphere of 5% CO₂ and 95% air at 37 ˚C.

Human papillary thyroid cancer cell lines (TPC-1, BCPAP), a human squamous thyroid cancer cell line (SW579), and a human follicular thyroid cancer cell line (FTC-133) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). A normal human thyroid cell line (Nthy-ori 3–1) was purchased from Jennio-Bio Co., Ltd. (Guangzhou, China). All cells were cultured in RPMI 1640 medium (Solarbio, Beijing, China) supplemented with 10% v/v foetal bovine serum (FBS, Gemini) and 1% v/v penicillin/streptomycin (Solarbio) in an atmosphere containing 5% CO₂ at 37°C. All cell lines used in our experiments have been checked by STR profiling, tested for mycoplasma contamination, and cultured at low passage.

A follicular thyroid carcinoma cell line (FTC-133), a normal thyroid epithelial cell line (Nthy-ori 3–1) and 293 T cells were acquired from Procell Life Science & Technology (Wuhan, China). These cell lines were cultured in DMEM or RPMI 1640 (Gibco, USA) with 10% heat-inactivated foetal bovine serum (FBS) (Gibco, USA), 100 U/mL penicillin, and 100 U/mL streptomycin. All cells were maintained at 37 °C in a humidified 5% CO₂ atmosphere cell incubator.