Akta pure 15 l instrument

Primers were purchased from Invitrogen and sequencing was done by Genewiz. All chemicals were purchased from Sigma Aldrich, Alfa Aesar, or Fisher Scientific without further purification. FPLC purification and analysis was performed using an AKTA pure 15 L instrument (UNICORN^™ software, GE Healthcare). ¹H NMR spectra was recorded on Burker Avance III 400MHz, calibrated using residual non-deuterated solvent as internal reference, processed by MestReNova (authorized to NYU). HRMS analyses were acquired on an Agilent 6224 Accurate-Mass time-of-flight LC/MS (LC-TOF) spectrometer with an electrospray (ESI) ionization source equipped with an autosampler. Molecular graphics and analyses were performed with PyMOL 2.5. GraphPad Prism software 9.0 was used for data analysis and plotting. pET His6 Sumo TEV LIC cloning vector (2S-T) was a gift from Scott Gradia (Addgene plasmid # 29711; http://n2t.net/addgene:29711; RRID:Addgene_29711).

All reagents were purchased from Sigma-Aldrich unless otherwise specified. FPLC separations were performed using an AKTA pure 15 L instrument (GE Healthcare). HPLC purification and analysis was performed on a Dionex UltiMate 3000 UHPLC+ (Thermo Scientific). For ATPase assays, ATP (Sigma Aldrich) and ADP (Promega) used in these assays were each 99% pure. Compound mass spectrometry analysis was performed on an Agilent 6224 TOF LC/MS, unless otherwise noted.