CH157 and IOMM cells were transfected with siRNA targeting the AMPK-α1 and α2 subunits (Santa Cruz, CA, USA) or scrambled siRNA (Santa Cruz, CA, USA) as a control using the Lipofectamine 2000 (RNAiMAX) Transfection Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. After transfection for 6 h, the culture medium was replaced with DMEM, followed by further studies.
Lipofectamine 2000 rnaimax transfection reagent
Manufactured by Thermo Fisher Scientific
Sourced in United States
Lipofectamine 2000 RNAiMAX is a transfection reagent designed for the delivery of siRNA and miRNA into a variety of cell types. It is formulated to efficiently deliver nucleic acids into the cells, enabling effective gene silencing.
Automatically generated - may contain errors
3 protocols using lipofectamine 2000 rnaimax transfection reagent
1
Silencing AMPK Subunits in Cancer Cells
2
Twist siRNA Knockdown Assay
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Small interfering RNA (siRNA) transfections were performed according to the manufacturer’s instructions using Lipofectamine 2000 RNAiMAX transfection reagent (Invitrogen). Twist siRNA (#sc38604, Santa Cruz Biotechnology, Dallas, TX, USA) and control siRNA (#sc37007, Santa Cruz Biotechnology, Dallas, TX, USA) were used to generate Twist knockdown (siTwist) and negative control (siNC) cells. The lyophilized siRNA duplex was reconstituted in RNase-free water to create 10 μM stock solutions. The transfected cells were confirmed by Western blot analysis.
3
HOXC6 Gene Silencing Protocol
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Briefly, AccuTarget™ Genome-wide predesigned HOXC6 siRNA (Bioneer Corp., Daeheon, Korea) and Lipofectamine 2000 RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA) were separately mixed in 150 μL of Opti-MEM medium (Gibro/Life Technology, Grand lsland, NY, USA). After incubation at room temperature for 5 min, diluted siRNA was added to dilute Lipofectamine® RNAiMAX Reagent (1:1 ratio). The cells were then incubated for 48 h at 37 °C in 5% CO2.
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