Embryos harvested at predetermined stages were dissected and fixed in fresh 4% paraformaldehyde overnight at 4°C. Samples were passed through a sucrose series, embedded in OCT (optimal cutting temperature) compound (Tissue-Tek), and sectioned on a cryostat at 8 μm. Staining was performed using either an RNAscope 2.5 HD Reagent Kit-RED assay (322350, Advanced Cell Diagnostics) or an RNAscope Fluorescent Multiplex Detection Reagent kit (320851, Advanced Cell Diagnostics) according to the manufacturer’s instructions. Fgf8 (313411), Tbx1 (481911), Dlx5 (478151), Lhx6 (422791), Gsc (504821), Nbl1 (454541), Hand1 (429651), Alx1 (403161), Ebf1 (433411), Gbx2 (314351), Maf (412951), Osr2 (517481), Pitx1 (587451), Lix1 (552041-C2), Cgnl1 (587441), Spry2 (425061), and Cdk1 (476081-C2) probes were designed and synthesized by Advanced Cell Diagnostics.
Rnascope fluorescent multiplex detection reagent kit
Manufactured by Advanced Cell Diagnostics
Sourced in United States
The RNAscope Fluorescent Multiplex Detection Reagent kit is a laboratory tool designed for the detection and visualization of targeted RNA molecules in tissue samples. The kit provides the necessary reagents and protocols to perform in situ hybridization and fluorescent signal amplification, enabling the simultaneous detection of multiple RNA targets within a single sample.
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Lab products found in correlation
2 protocols using rnascope fluorescent multiplex detection reagent kit
1
Embryonic Gene Expression Profiling
2
CXCL10 mRNA Expression in Retina
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Retinal frozen sections were incubated with mouse CXCL10 probe (Advanced Cell Diagnostics, Hayward, CA, USA) and sequentially hybridized to a cascade of signal amplification molecules from RNAscope Fluorescent Multiplex detection reagent kit (Advanced Cell Diagnostics) according to the manufacturer's instructions. At last, slides were counterstained with DAPI to label nuclei and viewed by epifluorescence microscope to detect CXCL10 mRNA-positive cells.
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