Nsc405020

The human trophoblast cell line HTR-8/SVneo and the human choriocarcinoma cell line JAR were initially obtained from Dr Charles H. Graham (Queen's University, Ontario, Canada) as previously described (Wang et al. 2008 (link), Dai et al. 2011) (link). HTR-8/SVneo and JAR cells were cultured in DMEM/F12 supplemented with 10% fetal bovine serum and 1% penicillin/ streptomycin and were incubated at 37°C in a humidified atmosphere containing 5% CO 2 . For proliferation assays, cells were incubated with different concentrations of recombinant LIF (0, 10, 25, 50 ng/mL; R&D Systems: 7734-LF) for 48 h. For migration and invasion assays, cells were cultured with different concentrations of recombinant human LIF (same concentrations as those used in the proliferation assay), a STAT3 inhibitor (5 µm; Selleck: S7501) or an MMP14 inhibitor (50 µm; NSC405020, Selleck: S8072) for 24 h. For the Western blot analysis, cells were incubated with rhLIF (10 ng/mL), a STAT3 inhibitor (5 µm) or an MMP14 inhibitor (50 µm) for 15 min.