Antibodies against icam 1

Antibodies against ICAM-1 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phosphorylated and total NF-κB p65 were obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against phosphorylated PKR T446, total PKR, Klotho, and β-actin were obtained from Abcam (Cambridge, MA, USA). Neutralizing antibodies against TLR2 (IMG-416E) and TLR4 (IMG-417E) were obtained from Novus Biologicals (Centennial, CO, USA). Medium 199 was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Recombinant human matrilin-2 and Klotho proteins (expressed by mouse myeloma cell line; endotoxin free) were obtained from R&D Systems (Minneapolis, MN, USA). C₁₃H₈N₄OS was obtained from MilliporeSigma (Burlington, MA, USA). Pam3CSK4 was obtained from InvivoGen (San Diego, CA, USA). Lipopolysaccharide (LPS, Escherichia coli 0111:B4), Bay11-7082, 2-Aminopurine, collagenase and other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Antibodies against ICAM-1, VCAM-1 and GAPDH were purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). Antibody against Klotho was purchased from Abcam Inc. (Cambridge, MA). Recombinant Klotho protein, recombinant IL-37 protein, and Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 were purchased from the R&D System (Minneapolis, MN). ELISA kit for Klotho was purchased from MyBioSource (San Diego, CA). Lipopolysaccharide (LPS, Escherichia coli 0111:B4) and all other chemicals and reagents were purchased from Sigma-Aldrich Chemical (St Louis, MO).

HaCaT cells were donated by Hyeonsook Cheong, Chosun University (Kwangju, Korea), and the cells were cultured in DMEM with 10% FBS (Gibco, New york, NY, USA), and tin protoporphyrin (Snpp; Calbiochem, Merck, Darmstadt, Germany). Recombinant human TNF-α and IFN-γ, and ELISA kits for IL-8, IL-6, and RANTES were obtained from BioLegend (San Diego, CA, USA). Antibodies against ICAM-1, FLG, IVL, actin, PCNA, HRP-conjugated anti-mouse, and anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against p-IκBα, IκBα, p65, p-stat1, p-stat3, HO-1, and Nrf2 were purchased from Cell Signaling Technology (Danvers, MA, USA). Other chemical reagents were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA).