The probes were dissolved in 50% dimethyl sulfoxide (DMSO) to a final concentration of 1 µg/µL and coated onto aldehyde group-modified glass slides (CapitalBio Corporation, Beijing, China) using SpotArray 72 (Perkin-Elmer Corporation, CA, USA). Each probe was spotted in triplicate. Coated slides were dried and stored at room temperature in the dark. Each glass slide contained eight individual arrays framed with an 8-sample cover slip containing individual reaction chambers. A schematic diagram of the probe positions on the microarray is shown in Fig. 1 .
Spotarray 72
Manufactured by PerkinElmer
Sourced in China, United States
The SpotArray 72 is a high-throughput microarray processing system designed for efficient sample processing and analysis. It provides automated hybridization, washing, and scanning of up to 72 microarray slides simultaneously, ensuring consistency and reproducibility in your research.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using spotarray 72
1
Probe Preparation for Microarray Analysis
2
Microarray Probe Immobilization on Aldehyde Slides
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The probes were dissolved in 50% dimethyl sulfoxide (DMSO) to a final concentration of 1 μg/μl and printed onto aldehyde group-modified glass slides (CEL Corporation, USA) using a SpotArray72 (Perkin-Elmer Corporation, CA, USA). Each probe was spotted in triplicate and each slide consisted of six microarrays framed with a 12 μl Geneframe (Beijing Capital Biochip Corporation, Beijing, China) which constituted individual reaction chambers. A schematic diagram of the probe positions of the single microarray is shown in Fig 1 .
3
Microarray-based Gene Expression Analysis
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Initially, 50 μl of sample was cultured on a fresh rabbit blood agar plate for 24 h. Growth was harvested for DNA extraction and analysis, as previously described [40 (link)]. In brief, microarrays were created by spotting oligonucleotide probes onto a glass slide using a SpotArray 7.2 (PerkinElmer). A two-step mPCR (to amplify the gene of interest and then label the PCR products) was conducted in two batches each. The resultant products were hybridised to a microarray slide for 16 h before being scanned (GenePix personal 4100A, Axon Instruments), and the signal intensity calculated with GenePix Pro 6.0.
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