Abc ap reagent

Manufactured by Vector Laboratories

The ABC-AP reagent is a laboratory product manufactured by Vector Laboratories. It is used for the detection and visualization of specific target molecules in biological samples. The reagent contains an alkaline phosphatase enzyme conjugate that can be used to amplify and enhance the signal from immunohistochemical or in situ hybridization assays.

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Lab products found in correlation

Avidin biotin solution, by Vector Laboratories (1 mentions) Biotinylated anti goat secondary antibody, by Vector Laboratories (1 mentions) Vector blue, by Vector Laboratories (1 mentions) Vs120 s6 w slide scanning microscope, by Olympus (1 mentions)

Close spelling variants of this product

ABC-AP Vector Blue Substrate reagents ABC-AP ABC reagent

2 protocols using abc ap reagent

Immunostaining of Macrophages in Visceral Fat

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For F4/80 immunostaining, paraffin sections of visceral fat post HFD were fixed in 10% paraformaldehyde and embedded as described in [29] (link). Sections were deparaffinized and then analyzed with F4/80 macrophage antibody as described previously [29] (link). Briefly, heat induced antigen retrieval was performed with 10 mM Citric Acid (pH 6.0). Sections were blocked with Avidin/Biotin solution (Vector Laboratories kit, #SP-2001) and 10% goat serum (Vector Laboratories kit, #SP-2001). The sections were incubated with primary antibody F4/80 (1∶500, AbD Serotec, #mcA497R) overnight at 4°C, followed by biotinylated anti-goat secondary antibody (1∶200, Vector Laboratories, #BA-9400) for 30 minutes, ABC-AP reagent (Vector Laboratories kit, #AK-5001) for 30 minutes, and vector red alkaline phosphate substrate (Vector Laboratories kit, #AK-5001) for 15 minutes and then counterstained with hematoxylin and eosin (H&E). Liver tissue was similarly fixed and embedded, and then sections were stained with H&E.

Johnston-Cox H., Eisenstein A.S., Koupenova M., Carroll S, & Ravid K. (2014). The Macrophage A2b Adenosine Receptor Regulates Tissue Insulin Sensitivity. PLoS ONE, 9(6), e98775.

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Immunohistochemical Analysis of TMEM97 in Human Eyes

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Eyes from individuals without a history or evidence of retinal disease upon gross examination were obtained within 12 h of death and preserved in formalin and embedded in paraffin. Five-micron thick sections were prepared for immunohistochemistry by bleaching pigment using oxalic acid/potassium permanganate solution followed by heat-induced epitope retrieval with Tris-EDTA buffer (pH 9) at 60 °C for 1 h. Immunohistochemical staining of bleached eye sections was performed with rabbit anti-human TMEM97 antibody (Proteintech Cat. No. 26444–1-AP, diluted 1:100 in DAKO antibody diluent) overnight at 4 °C. Rabbit IgG isotype control was applied to adjacent sections in place of the primary antibody to ascertain the specificity of staining. Slides were incubated with a biotin-conjugated secondary antibody, followed by incubation with VECTASTAIN® ABC-AP reagent and development using Vector Blue (Vector Laboratories, Cat No. AK-5000 and SK-5300, respectively). Images were obtained using an Olympus VS120-S6-W slide-scanning microscope.

Shen H., Li J., Heisler-Taylor T., Makin R., Yang H., Mavlyutov T.A., Gelfand B., Cebulla C.M, & Guo L.W. (2021). TMEM97 ablation aggravates oxidant-induced retinal degeneration. Cellular signalling, 86, 110078.

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