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Poly maleic anhydride alt 1 octadecene pmao

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Poly(maleic anhydride-alt-1-octadecene) (PMAO) is a synthetic polymer produced through the alternating copolymerization of maleic anhydride and 1-octadecene. It is a white to off-white solid material with a high molecular weight. PMAO can be used in various industrial applications, but its core function is as a chemical intermediate without further interpretation.

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4 protocols using poly maleic anhydride alt 1 octadecene pmao

1

Synthesis and Characterization of Quantum Dots

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Copper (I) acetate (97 %), zinc acetate (99 %), oleic acid (90 %), oleylamine (70 %), 1-octadecene (ODE, 90 %), poly(maleic anhydride-alt-1-octadecene) (PMAO) (average Mn 30,000–50,000 as a powder), 3-(dimethylamino)-1-propylamine (DMAPA) (99 %), methyl iodide (99 %), poly-D-lysine, and gelatin were purchased from Sigma-Aldrich. Indium (III) acetate (99.99 %) and 1-dodecanethiol (DDT, 98 %) were purchased from Alfa Aesar. RPMI-1640 and MEM media were from Corning Cellgro. Heat-inactivated fetal bovine serum(FBS) was from Gibco. Fluorescein diacetate/propidium iodide (FDA/PI) was from Invitrogen. Dulbecco’s phosphate-buffered saline (DPBS) was from Fisher Scientific. U-87 MG and HEK-293 cells were ordered from ATCC. Chemicals were used as received without further purification.
The ultraviolet and visible (UV–Vis) spectra of QDs in organic solvents and aqueous solutions were obtained with a UV–Vis spectrometer (UV-2450 from Shimadzu) using cuvettes with a path length of 1 cm. Photoluminescence spectra were acquired using a spectrophotometer (RF-5301PC from Shimadzu). Infrared (IR) spectra of polymers and QDs were collected using a FT-IR spectrometer (Perkin-Elmer Frontier) equipped with attenuated total reflectance and measurement software (Spectrum 10). Particle size measurements of QDs were made using a ZetaPALS dynamic light scattering detector (Brookhaven Instruments Corp.).
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2

Multifunctional Nanoparticle-Mediated Drug Delivery

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All the chemical reagents were of analytical grade and were used without additional purification. Iron (III) acetylacetonate (99%), squalene (98%), dibenzyl ether (99%), decanoic acid (99%), 2-(N-Morpholino)ethanesulfonic acid (MES) (99%), MTT Formazan powder, and Doxorubicin hydrochloride (DOX) (98%) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). KMnO4 and poly (maleic anhydride-alt-1-octadecene) (PMAO, MW = 30,000–50,000 g mol−1) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Fetal bovine serum was obtained from AusgeneX Pty Ltd. (Gold Coast, Australia). RPMI-1640, penicillin, and streptomycin were obtained from HyClone (GE, United States). Ultrapure water (18.2 mΩ cm−1) generated using a Millipore Milli-Q purification system.
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3

Multiplex Mycotoxin Detection Assay

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Aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), zearalenone (ZEN), citrinin (CIT), FB1, and ochratoxin A (OTA) were purchased from Huaan Magnech Bio-Tech Co., Ltd. (Beijing, China). Oleylamine, sodium dodecyl sulfonate (SDS), gold(III) chloride hydrate, trisodium citrate (Na3C6H5O7·2H2O), bovine serum albumin (BSA), 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC), and poly (maleicanhydride-alt-1-octadecene) (PMAO, MW = 30,000~50,000 Da) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-FB1 monoclonal antibodies (mAbs) were kindly provided by prof. Yang Xu from Nanchang University (Nanchang, China). The BSA-FB1 conjugates (molar ratio of 1:23) were prepared by our laboratory. The sample pad, the absorbent pad, and the NC membrane were provided by Wuxi Zodolabs Biotech Co., Ltd. (Jiangsu, China). Goat anti-mouse IgG was obtained from Chongqing Xinyuanjiahe Biotechnology Inc. (Chongqing, China). Other chemicals were of analytical grade and purchased from Sinopharm Chemical Corp. (Shanghai, China). All reagents were used without further purification.
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4

Organic Solvent Synthesis of Nanoparticles

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Calcium chloride (CaCl 2 ), sodium carbonate (Na 2 CO 3 ), 5 wt% aqueous sodium hypochlorite (NaClO) solution, tetrahydrofran (THF) and HPLC-grade methanol were purchased from Kanto Chemical (Tokyo, Japan). Toluene, acetone, potassium hydroxide (KOH), trifluoroacetic acid (TFA), oleic acid, sodium tetraborate decahydrate (Na 2 B 4 O 7 Á10H 2 O), sodium hydroxide (NaOH), naphthalene and clomipramine hydrochloride were purchased from Wako Pure Chemical (Osaka, Japan). Poly(sodium 4-sulfonate) (PSS, M w : B70 000) and poly(maleic anhydride-alt-1-octadecene) (PMAO, M n : 30 000-50 000) were purchased from Sigma-Aldrich (St. Louis, MO). Benzene, methylbenzene, ethylbenzene, amylbenzene, butylbenzene, hexylbenzene, heptylbenzene, n-octylbenzene, nonylbenzene, decylbenzene and imipramine hydrochloride were purchased from Tokyo Chemical Industry (Tokyo, Japan). A 3 mm fully porous ODS silica gel (CHEMCOSORB 3-ODS-L) was purchased from Chemco Scientific (Osaka, Japan). Porcine whole blood with 0.3 wt% citric acid was purchased from Tokyo Shibaura Zouki CO (Tokyo, Japan). Ultrapure water (18.2 MO cm) was obtained from a PURELAB flex water purification system (ELGA, Veolia Water, Marlow, U.K.).
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