Vsv g p5d4

Antibodies reactive to Bcl-rambo (Rocky-1; Santa Cruz Biotechnology, Santa Cruz, CA, USA), cytochrome c (7H8.2C12; BD Biosciences, San Jose, CA, USA), FLAG (1E6, Wako Pure Chemical Industries, Osaka, Japan), HSP60 (insect) (Enzo Life Science, Farmingdale, NY, USA), α-tubulin (DM1A; Sigma-Aldrich, St. Louis, MI, USA), and VSV-G (P5D4; Santa Cruz Biotechnology) were commercially obtained. Antibodies reactive to Cut (2B10), Discs large (4F3), Elav (7E8A10), and Prospero (MR1A) were obtained from the Developmental Studies Hybridoma Bank (Iowa City, IA, USA). Secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Jackson ImmunoResearch (West Grove, PA, USA).

Mouse monoclonal antibodies to FLAG (IE6; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), VSV-G (P5D4; Santa Cruz Biotechnology, Dallas, TX, USA), the VSV glycoprotein (P5D4, SAB4200695; Sigma-Aldrich), GRP75 (D-9; Santa Cruz Biotechnology), cytochrome c (7H8.2C12; BD Biosciences, Franklin Lakes, NJ, USA), PARP-1 (C2-10; R &D Systems, Minneapolis, MN, USA), γ1-actin (2F3; FUJIFILM Wako Pure Chemical Corporation), β-actin (AC-15; Sigma-Aldrich), and GAPDH (6C5; Santa Cruz Biotechnology), a rat monoclonal antibody to caspase-7 (11E4; Sigma-Aldrich), and a rabbit polyclonal antibody to HSP60 (insect, ADI-SPA-805; Enzo Life Sciences, Farmingdale, NY, USA) were used as primary antibodies. Peroxidase-conjugated goat anti-mouse IgG (H + L) (115-035-146; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), goat anti-rabbit IgG (H + L) (111-035-144; Jackson ImmunoResearch Laboratories), and goat anti-rat IgG (H + L) (J112-035-143; Jackson ImmunoResearch Laboratories) were used as secondary antibodies.