The antibodies against the EGFR (D38B1) uncoupled or coupled to sepharose beads or to Alexa Flour (AF) 488, the phospho-EGFR-Y1068 (D7A5) and -Y1045 (#2237), -T669 (#8808) and the K48-polyubiquitin (12805), as well as the rabbit IgG isotype control (DA1E) were from Cell Signaling. The anti-EEA1 (BD Clone 14) and CD63 (BD clone H5C6) were purchased from BD Biosciences and M2-FLAG-affinity Gel and antibodies from Sigma and the GFP-tag antibody from Thermo-Scientific (A-11122). The guinea pig polyclonal antibody against HPV8E6 was kindly provided by Janet L. Brandsma and is described in Hufbauer et al. (2010) (link).
Gfp tag antibody
Manufactured by Thermo Fisher Scientific
The GFP-tag antibody is a laboratory reagent used for the detection and analysis of proteins tagged with the green fluorescent protein (GFP) in biological samples. The antibody specifically recognizes and binds to the GFP tag, allowing visualization and quantification of GFP-tagged proteins.
Automatically generated - may contain errors
2 protocols using gfp tag antibody
1
EGFR Signaling Pathway Analysis
2
Whole-mount immunolocalization of GFP
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An improved procedure for whole-mount immunolocalization was adopted. In brief, 1 cm root pieces were mounted in 2% formaldehyde in microtubule-stabilizing buffer (MTSB) supplemented with 0.1% Triton X-100. Vacuum infiltration of explants, hydrophilization, cell wall digestion and membrane permeabilization were performed as described previously (Pasternak et al., 2015 (link)). After 3 h of blocking in BSA, samples were incubated with a GFP Tag antibody (1:200 dilution) coupled to Alexa Fluor 488 (Thermo Fisher Scientific) for 1 h at room temperature, followed by three washes and then mounted in Slow FadeTM Diamond anti-fade mountant for confocal microscopy.
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