Total RNA of OC samples was extracted using TriZol Reagent (Takara, Japan). cDNA synthesis was carried out using the AMV reverse transcriptase reagent box (Takara, Japan). Real-time PCR was performed using a 2 × SYBR Green PCR Master Mix. Next, the 2-ΔΔCt method was used to calculate the relative gene expression with GAPDH serving as an internal reference. The sequences of primers used for RT-qPCR are presented in Supplementary Table 2 .
Amv reverse transcriptase reagent box
Manufactured by Takara Bio
Sourced in Japan
The AMV reverse transcriptase reagent box contains the necessary components for the reverse transcription of RNA into complementary DNA (cDNA). The box includes the AMV (Avian Myeloblastosis Virus) reverse transcriptase enzyme, which catalyzes the conversion of RNA to single-stranded cDNA. This product is designed for use in molecular biology applications that require the synthesis of cDNA from RNA templates.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using amv reverse transcriptase reagent box
1
Quantitative Gene Expression Analysis
2
Extraction and Analysis of lncRNA in Ovarian Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We extracted the total RNA of 54 OC clinical samples using TriZol Reagent (Takara, Japan). the AMV reverse transcriptase reagent box (Takara, Japan) was used to carry out the cDNA synthesis. A 2 × SYBR Green PCR Master Mix was used to perform Real-time PCR. Next, GAPDH was served as an internal reference and the 2-ΔΔCt method was used to calculate the relative gene expression of the lncRNAs in risk model. The sequences of primers of the lncRNAs are presented in Supplementary Table 1 .
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