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Phospho acetyl coa carboxylase

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Phospho-Acetyl-CoA Carboxylase is a lab equipment product that detects the phosphorylation state of Acetyl-CoA Carboxylase. Acetyl-CoA Carboxylase is an enzyme that plays a key role in fatty acid synthesis.

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Lab products found in correlation

Acetyl coa carboxylase, by Cell Signaling Technology (3 mentions) Phospho ampkα, by Cell Signaling Technology (3 mentions) Fatty acid synthase, by Cell Signaling Technology (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) 3 n morpholino propanesulfonic acid buffer, by Thermo Fisher Scientific (1 mentions) Pt172 ampkα, by Cell Signaling Technology (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Ampkβ, by Cell Signaling Technology (1 mentions) N cadherin, by Santa Cruz Biotechnology (1 mentions) Phospho smad2 3, by Cell Signaling Technology (1 mentions) Total acetyl coa carboxylase, by Cell Signaling Technology (1 mentions) Total oxphos human wb antibody cocktail, by Abcam (1 mentions) Hexokinase 2, by Abcam (1 mentions) Lactate dehydrogenase a, by Cell Signaling Technology (1 mentions) Stearoyl coa desaturase 1, by Cell Signaling Technology (1 mentions) Total smad2 3, by Cell Signaling Technology (1 mentions) Recombinant human tnf α, by R&D Systems (1 mentions) Snail, by Cell Signaling Technology (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Acetyl-CoA Carboxylase (66 citations) Phospho-Acetyl-CoA Carboxylase (Ser79) (16 citations) Anti-phospho-acetyl-CoA carboxylase (ACC), (11 citations) Rabbit anti-phospho-Acetyl-CoA Carboxylase (Ser79) (3 citations) Phospho-Acetyl-CoA carboxylase (Ser79) (pACC (2 citations) Phospho-Acetyl-CoA carboxylase (p-ACC) (2 citations) Anti-phospho acetyl-CoA carboxylase (p-ACC) (2 citations)

7 protocols using phospho acetyl coa carboxylase

1

Western Blot Analysis of Metabolic Proteins

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Proteins were resolved on 10–12% (w/v) NuPAGE BisTris gels in 3‐(N‐morpholino)propanesulfonic acid buffer (Life Technologies) and transferred to polyvinylidene fluoride membrane. Proteins were analysed by western blot using the following antibodies: phospho‐acetyl‐CoA carboxylase, AMPKα (pT172) and AMPKβ (all at 1 : 1000; Cell Signaling Technology, Beverly, MA, USA).
Rousset C.I., Leiper F.C., Kichev A., Gressens P., Carling D., Hagberg H, & Thornton C. (2015). A dual role for AMP‐activated protein kinase (AMPK) during neonatal hypoxic–ischaemic brain injury in mice. Journal of Neurochemistry, 133(2), 242-252.
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2

Molecular Mechanisms of EMT and Metabolism

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Recombinant human TNFα and TGFβ were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies used are listed as follows: IkB, phospho-Smad2/3, total-Smad2/3, Snail, vimentin, lactate dehydrogenase-A, fatty acid synthase, stearoyl-CoA desaturase-1, phospho-acetyl-CoA carboxylase, total-acetyl-CoA carboxylase (Cell signaling, MA, USA); beta-actin, E-cadherin, N-cadherin (Santa Cruz, TX, USA); hexokinase II, pyruvate kinase isoform 2, fructose-bisphophotase-2, total OXPHOS Human WB Antibody Cocktail (Abcam, Cambridge, UK); Zeb1 (ProSci, CA, USA); and fructose-bisphophotase-1 (Abgent, Wuxi, China). All other reagents were from Sigma-Aldrich (MO, USA) unless stated otherwise.
Liu M., Quek L.E., Sultani G, & Turner N. (2016). Epithelial-mesenchymal transition induction is associated with augmented glucose uptake and lactate production in pancreatic ductal adenocarcinoma. Cancer & Metabolism, 4, 19.
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3

Western Blot Analysis of Metabolic Proteins

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Whole cell protein extracts were prepared using RIPA lysis buffer 30–50 μg total protein were size-separated on SDS-PAGE and transferred to PVDF. Blots were probed with antibodies to FASN (Antibody #3189), phospho-ATP-Citrate Lyase (Ser455; P-ACLY, Antibody #4331), ATP-Citrate Lyase (ACLY; Antibody #43320), phospho-Acetyl-CoA Carboxylase (Ser79; P-ACC, Antibody #3661), Acetyl-CoA Carboxylase (ACC; Antibody #3676), phospho-AMPKα (Thr172; P-AMPK, Antibody #2531), AMPKα (AMPK; Antibody #2532), PARP (Antibody #9542), or IGF-I Receptor β (IGF1R; Antibody #3018) which were all obtained from Cell Signaling Technology, Inc. (Beverly MA). Antibodies to β-actin (AC-75), GAPDH (Clone GAPDH-71.1), and alpha tubulin (clone B-5-1-2) and secondary antibody rabbit anti-mouse HRP were obtained from Sigma Aldrich Co. PSTAIR antibody was obtained from Abcam (Cambridge, MA). Goat anti- rabbit HRP was obtained from MP Biomedicals. Goat anti-mouse or rabbit immunoglobulin labelled with Alexa Fluor® 680 was obtained from Molecular Probes, (Eugene, OR). For quantitation, blots were scanned using an Odyssey Infrared Imager (LI-COR Biosciences, Lincoln, NE), and FASN signal normalized to GAPDH expression.
Wahdan-Alaswad R.S., Cochrane D.R., Spoelstra N.S., Howe E.N., Edgerton S.M., Anderson S.M., Thor A.D, & Richer J.K. (2014). Metformin-Induced Killing of Triple-Negative Breast Cancer Cells Is Mediated by Reduction in Fatty Acid Synthase via miRNA-193b. Hormones & Cancer, 5(6), 374-389.
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4

Western Blot Analysis of Cell Signaling

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Cell lysates were prepared using a kit from Cell Signaling (Beverly, MA, USA). Equal amounts of protein (determined using the Bio-Rad Protein assay) were run on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and transferred to polyvinylidene difluoride membranes, which were washed with Tris-buffered saline (TBS) containing 0.1% Tween 20 (TBST), blocked with TBST and 5% wt/vol nonfat dry milk overnight at 4℃, and incubated with the following primary antibodies: CDK2, β-actin, CyclinD1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) and phospho-mTOR (Ser2448), phospho-mTOR (Ser2481), phospho-p70S6K (Thr389), phospho-4E BP1 (Thr37/46), phospho-AMPKα (Thr172), phospho-Acetyl-CoA carboxylase (Ser79), phospho-extracellular signal–regulated kinase (Thr202/Tyr204), phospho-AKT (Ser473) (Cell Signaling). Membranes were then washed with TBST and incubated with horseradish peroxidase-conjugated secondary antibody (1:1,000). Signals were detected using an Amersham enhanced chemiluminescence (GE Healthcare Life Sciences, Munich, Germany).
Ko Y., Choi A., Lee M, & Lee J.A. (2016). Metformin displays in vitro and in vivo antitumor effect against osteosarcoma. Korean Journal of Pediatrics, 59(9), 374-380.
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5

Western Blot Analysis of Signaling Proteins

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HRMECs or tissues were lysed by lysis buffer (Cell Signaling, Topsfield, MA, USA). Total protein (50 μg) was loaded on Bio-Rad 4–20% gel system, transferred to PVDF membrane, and immunoblotted with one of the following primary antibodies: Serine473 phosphorylated Akt (Cat#4060, Cell Signaling), total Akt (Cat#4691,Cell Signaling), Threonine172 phosphorylated AMPK (Cat#2535, Cell Signaling), total AMPK (Cat#5832, Cell Signaling), Acetyl-CoA Carboxylase (Cat #3662, Cell Signaling), Phospho-Acetyl-CoA Carboxylase (Cat #11818, Cell Signaling), Claudin-1 (Cat#13995, Cell Signaling), zo-1 (Cat#13663, Cell Signaling), Claudin-5 (Cat#35-2500, Thermo-Fisher Scientific), Occludin (Cat#71-1500, Thermo-Fisher Scientific), and ß-Actin (Cat#SC-4778, Sigma). The secondary antibody was obtained from Cell Signaling Company. The membrane was exposed to Super-Signal Reagent (Pierce, IL, USA), and imaged on a Bio-Rad ChemiDoc Touch station (Bio-Rad).
Yan Z., Wang C., Meng Z., Gan L., Guo R., Liu J., Bond Lau W., Xie D., Zhao J., Lopez B.L., Christopher T.A., Naik U.P., Ma X, & Wang Y. (2022). C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions. Cells, 11(5), 779.
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6

Regulatory T cell Signaling Analysis

Cited 1 time
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CD4+YFP+ Treg cells and CD4+YFP− conventional T cells were sorted from Foxp3Cre and Foxp3CreLkb1f/f mice and treated as indicated, and then cells were lysed and western blot was carried out as previously described55 (link) with antibodies to Lkb1 (D60C5, Cell Signalling Technology), phosphorylated AMPK (40H9, Cell Signalling Technology), IKKβ (2C8, Cell Signalling Technology), phosphorylated IKKα/β (16A6, Cell Signalling Technology), phosphorylated IκBα (14D4, Cell Signalling Technology), phospho-NF-κB p65 (93H1, Cell Signalling Technology), NF-κB p65 (D14E12, Cell Signalling Technology), STAT4 (C46B10, Cell Signalling Technology), AMPKα (D63G4, Cell Signalling Technology), phospho-Acetyl-CoA Carboxylase (D7D11, Cell Signalling Technology), phospho-STAT4 (Abcam), TGF-βR2 (Abcam) and GAPDH (D16H11, Cell Signalling Technology).
Wu D., Luo Y., Guo W., Niu Q., Xue T., Yang F., Sun X., Chen S., Liu Y., Liu J., Sun Z., Zhao C., Huang H., Liao F., Han Z., Zhou D., Yang Y., Xu G., Cheng T, & Feng X. (2017). Lkb1 maintains Treg cell lineage identity. Nature Communications, 8, 15876.
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7

Comprehensive Western Blot Antibody Panel

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The following primary antibodies were used for western blotting at 1:1000 dilution: Ago2 (MBL CM004-3), Ago1 (MBL RN028PW), Glucokinase (Abcam ab88056), β-Actin (Sigma A1978), GAPDH (Abcam ab8245), α-Tubulin (Sigma, T6557), Glucose-6-phosphatase (Abcam ab83690), Glycogen synthase (Cell Signaling, #3893), Insulin receptor (Cell Signaling, #3025), Akt2 (Cell Signaling, #2964), phospho-Akt2 (Ser474) (Cell Signaling, #8599), FoxO1 (Cell Signaling, #2880), phospho-FoxO1 (Cell Signaling, #9464), Acetyl-CoA Carboxylase (Cell Signaling, #3676), phospho-Acetyl-CoA Carboxylase (Cell Signaling, #11818), Fatty Acid Synthase (Cell Signaling, #3180), AMPKα (Cell Signaling, #2532), and phospho-AMPKα (Cell Signaling, #2535). Image densitometry of 16-bit TIF images for all western blots was performed using ImageJ.
Yan X., Wang Z., Bishop C.A., Weitkunat K., Feng X., Tarbier M., Luo J., Friedländer M.R., Burkhardt R., Klaus S., Willnow T.E, & Poy M.N. (2018). Control of hepatic gluconeogenesis by Argonaute2. Molecular Metabolism, 18, 15-24.
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