The human tumor cell lines MKN1, HT-1080 (Health Science Research Resources Bank), HeLa S3, and MDA-MB-231 (ATCC) were cultured in DMEM supplemented with 10% FBS. Cell motility was analyzed with a transwell migration assay as described previously (Tanimura et al., 2011 (link)) as well as with a wound-healing assay. In the latter, a scratch wound was introduced in a monolayer of MKN1 cells with a plastic micropipette tip. After incubation for 8 h to allow wound healing, cells were fixed with 100% methanol and stained with Coomassie brilliant blue. Images were acquired with an Axiovert 200M microscope (ZEISS) equipped with an AxioCam MRm camera (ZEISS), and the width of the wound area was measured with the use of AxioVision software version 4.8.2 (ZEISS).
Ht 1080
Manufactured by Health Science Research Resources Bank
Sourced in Japan
The HT-1080 is a laboratory equipment that serves as a cell culture incubator. It is designed to maintain a controlled environment for the growth and cultivation of cells, providing the necessary temperature, humidity, and atmospheric conditions to support cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Axiovert 200m microscope, by Zeiss (1 mentions)
Axiovision software version 4, by Zeiss (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Axiocam mrm camera, by Zeiss (1 mentions)
Hela s3, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Minimum essential medium (mem), by Merck Group (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Hmscs, by Lonza (1 mentions)
2 protocols using ht 1080
1
Cell Motility Assay for Tumor Cell Lines
2
Culturing Cancer and Stem Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human cervical cancer cell line HeLa, human sarcoma cell line HT-1080, and human glioblastoma cell lines U87 and U251 were obtained from the Health Science Research Resources Bank (Osaka, Japan). These cell lines were maintained in Eagle’s minimum essential medium (MEM; Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS; Sigma), 100 U/ml penicillin and 100 μg/ml streptomycin (Nacalai Tesque, Kyoto, Japan). hMSCs were purchased from Lonza and cultured in MSCGMTM medium (Lonza, Basel, Switzerland). Cells were cultured in a humidified atmosphere of 5% CO2 and 95% air at 37 °C and were passaged upon reaching 80% confluence.
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