Matrigel fibronectin coated

Manufactured by Merck Group

Sourced in France

Matrigel/fibronectin-coated is a specialized cell culture substrate used for the attachment and growth of various cell types. It is a laminin-rich extracellular matrix (ECM) preparation derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. Fibronectin is an adhesive glycoprotein that promotes cell attachment. This coated substrate provides a more physiologically relevant environment for cell culture applications.

Automatically generated - may contain errors

Lab products found in correlation

Doxycycline, by Merck Group (1 mentions) Mopc 21, by Abcam (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions)

Close spelling variants of this product

Matrigel-fibronectin Fibronectin-coated Matrigel-coated Fibronectin Matrigel

3 protocols using matrigel fibronectin coated

Modulating CD36 Expression in Pancreatic Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS-1 cells carrying the Tet-on system for CD36 overexpression (15 (link)) were cultured with RPMI 1640 medium containing 11.1 mmol/L glucose, 10% FBS, 100 IU/mL penicillin, 100 μg/mL streptomycin, 50 mg/mL neomycin, 50 mg/mL hygromycin, 10 mmol/L HEPES, 1 mmol/L sodium pyruvate, and 50 μmol/L β-mercaptoethanol at 37°C in a humidified atmosphere with 5% CO₂. To induce CD36 expression, cells were seeded in 24- or 48-well plates and cultured with or without 500 ng/mL doxycycline (Sigma-Aldrich, St. Louis, MO) for 72 h.
EndoC-βH1 cells (16 (link)) were cultured in Matrigel/fibronectin-coated (100 μg/mL and 2 μg/mL, respectively) (Sigma-Aldrich) vessels with DMEM containing 5.6 mmol/L glucose, 2% BSA, 10 mmol/L nicotinamide, 50 μmol/L β-mercaptoethanol, 5.5 μg/mL transferrin, 6.7 ng/mL sodium selenite, 100 IU/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere with 5% CO₂. Cells were seeded in Matrigel/fibronectin-coated 48-well plates and cultured for 72 h with 2 μg/mL of a CD36-blocking antibody (FA6.125, catalog number 60084; STEMCELL Technologies, Vancouver, British Columbia, Canada) or an isotype control (MOPC-21, catalog number ab18443; Abcam, Cambridge, U.K.).

Nagao M., Esguerra J.L., Asai A., Ofori J.K., Edlund A., Wendt A., Sugihara H., Wollheim C.B., Oikawa S, & Eliasson L. (2020). Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells. Diabetes, 69(6), 1193-1205.

+ Open protocol

+ Expand

Isolation and Culture of Rodent Pancreatic Islets and Beta Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Goto‐Kakizaki (GK/LU colony, bread in‐house)¹⁶ and Wistar control rats (Taconic) age 11–14 weeks were sacrificed in a CO₂ chamber and the islets were isolated using collagenase digestion as previously described.²⁵ All animal procedures were approved by the Malmö/Lund Committee for Animal Experiment Ethics, Sweden.
INS‐1 832/13 cells³² were maintained in RPMI 1640 medium (HyClone, UT, USA) containing 11.1 mM D‐glucose (HyClone, UT, USA), supplemented with 10% (v/v) heat inactivated fetal bovine serum (FBS), 100 IU/ml of penicillin (HyClone, UT, USA), 100 μg/ml of streptomycin (HyClone, UT, USA), 10 mM HEPES (HyClone, UT, USA), 2 mM L‐glutamine (HyClone, UT, USA), 1 mM sodium pyruvate (HyClone, UT, USA), and 50 μM 2‐mercaptoethanol. Glucolipotoxic conditions were induced by incubating the cells in 16.7 mM glucose and 0.5 mM palmitate for 48 h.
EndoC‐βH1 cells (EndoCells, Paris, France)³³ were seeded in matrigel/fibronectin‐coated (100 μg/ml and 2 μg/ml, respectively, Sigma‐Aldrich) culture vessels in DMEM containing 5.6 mmol/L glucose, 2% BSA fraction V, 10 mmol/L nicotinamide, 50 μmol/L 2‐mercaptoethanol, 5.5 μg/ml transferrin, 6.7 ng/ml sodium selenite, 100 U/ml penicillin, and 100 μg/ml streptomycin.
All cells were incubated in a humidified atmosphere with 5% CO₂ at 37°C.

Ofori J.K., Karagiannopoulos A., Barghouth M., Nagao M., Andersson M.E., Salunkhe V.A., Zhang E., Wendt A, & Eliasson L. (2022). The highly expressed calcium‐insensitive synaptotagmin‐11 and synaptotagmin‐13 modulate insulin secretion. Acta Physiologica (Oxford, England), 236(1), e13857.

+ Open protocol

+ Expand

Culturing of INS1 832/13 and EndoC-βH1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS1 832/13 and EndoC-βH1 cells were cultured as previously described [22 (link),23 (link)]. EndoC-βH1 cells were cultured in Matrigel/fibronectin-coated (100/2 mg/mL, Sigma-Aldrich) flasks with DMEM containing 5.6 mmol/L glucose, 2% BSA, 10 mmol/L nicotinamide, 50 mmol/L β-mercaptoethanol, 5.5 mg/mL transferrin, 6.7 ng/mL sodium selenite, 100 IU/mL penicillin, and 100 mg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO₂.

Cataldo L.R., Gao Q., Argemi-Muntadas L., Hodek O., Cowan E., Hladkou S., Gheibi S., Spégel P., Prasad R.B., Eliasson L., Scheele C., Fex M., Mulder H, & Moritz T. (2022). The human batokine EPDR1 regulates β-cell metabolism and function. Molecular Metabolism, 66, 101629.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!