Tetrahydrofuran thf

Lipase B form Candida antarctica (lipozyme CalB) was a kind gift from Novozymes (Bagsværde, Denmark). CalB immo Plus was purchased from c-LEcta, Germany. Copper bromide, N,N,N′,N″,N″-pentamethyldiethylenetriamine, methyl methacrylate (MMA), 4-methylumbelliferyl butyrate, oleic acid (88%), and tetramethyl orthosilicate were purchased from Sigma Aldrich/Merck (Darmstadt, Germany)). 3-Aminopropyltriethoxysilane and 3-(trimethoxysilyl)propyl 2-bromo-2-methylpropanoate were from abcr (Karlsruhe, Germany). Acetonitrile, ascorbic acid, anisole, ethanol (abs.), glutaraldehyde (25%), isopropyl alcohol, methanol, n-hexane, tetrahydrofuran (THF), and pyridine were from Carl Roth (Karlsruhe, Germany). MMA was purified using an aluminum oxide column followed by distillation before polymerization and isopropyl alcohol was dried with molecular sieves. All other chemicals were used as received.

Pyridine (> 99%, distilled before use), ethyl acetate (> 99%), and platinum(IV) oxide (PtO₂) were purchased from Sigma-Aldrich (Steinheim, Germany), whereas cyclohexane (> 99%), methanol, and n-hexane (both HPLC grade) were from Th. Geyer (Renningen, Germany). cyclohexane and ethyl acetate were combined (1:1, v/v) and distilled to give the azeotropic mixture CE_az (46:54, w/w). Tetrahydrofuran (THF) (> 99%) (distilled and stored over a 3-Å molecular filter before use), sulfuric acid (96%), anhydrous sodium sulfate, and sodium chloride (> 99.5%) were from Carl Roth (Karlsruhe, Germany). Silylation agent N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was from Macherey–Nagel (Düren, Germany) and helium, hydrogen (both 5.0 quality), and nitrogen (99.95%) were from Westfalen company (Münster, Germany). Demineralized water was prepared in-house. An internal standard solution of docosanoic acid methyl ester (22:0-ME) in n-hexane with a concentration of 5 μg/mL was prepared after docosanoic acid was transesterified with 1% sulfuric acid in methanol according to Wendlinger and Vetter [26 (link)]. Whole rye grains were obtained in a local supermarket in Stuttgart (Germany).

Polystyrene-block-polyacrylic acid (PS-b-PAA, batch: MKBQ5839V) was purchased from Sigma Aldrich (Steinheim, Germany). The degree of polymerization of PS is 275 and of PAA 30, as stated by the manufacturer. The molecular weights (M_n) and the polydispersity index (PDI) of PS-b-PAA are summarized in Table 1.
Deionized water was used as the selective solvent for the hydrophobic part (polystyrene). Tetrahydrofuran (THF, for HPLC, not stabilized) and 1,4-dioxane (for HPLC, not stabilized) from Carl Roth (Karlsruhe, Germany) were utilized as good solvents for PS-b-PAA.
Filters were used for microscopy (Whatman^® Nuclepore™ track-etched polycarbonate membranes, with pore diameters of between 0.2 µm and 2.0 µm, Dassel, Germany). A quartz glass cuvette (Hellma Analytics, Type 100 QS, Müllheim, Germany) with a layer thickness of 10 mm and a nominal volume of 3.5 mL was used for dynamic light scattering measurements.