The Fireplex Multiplex Circulating MicroRNA Assay (Abcam, MA) was used for direct quantification of 58 miRs from plasma collected with heparin. miRs were hybridized to complementary oligonucleotides covalently attached to encoded hydrogel microparticles. The bound target was ligated to oligonucleotide adapter sequences that serve as universal polymerase chain reaction priming sites. The miR–adapter hybrid models were then denatured from the particles, and reverse transcription polymerase chain reaction was performed using a fluorescent forward primer. Once amplified, the fluorescent target was rehybridized to the original capture particles and scanned on an EMD Millipore Guava 6HT flow cytometer (Merck KGaA Darmstadt, Germany).
Emd millipore guava 6ht flow cytometer
Manufactured by Merck Group
Sourced in Germany
The EMD Millipore Guava 6HT flow cytometer is a compact, benchtop instrument used for cell analysis. It employs flow cytometry technology to measure and analyze various properties of cells or particles in a fluid sample. The core function of the Guava 6HT is to provide rapid, quantitative, and multiparameter analysis of cells.
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Lab products found in correlation
2 protocols using emd millipore guava 6ht flow cytometer
1
Plasma miRNA Profiling via Fireplex
2
Quantitative Plasma miRNA Profiling
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The Fireplex Multiplex Circulating MicroRNA Assay (Abcam, MA) was used for direct quantification of 58 miRNAs from plasma. MiRNAs were hybridized to complementary oligonucleotides covalently attached to encoded hydrogel microparticles. The bound target was ligated to oligonucleotide adapter sequences that serve as universal PCR priming sites. The miR-adapter hybrid models were then denatured from the particles and reverse transcription polymerase chain reaction (RT-PCR) was performed using a fluorescent forward primer. Once amplified, the fluorescent target was rehybridized to the original capture particles and scanned on an EMD Millipore Guava 6HT flow cytometer (Merck KGaA Darmstadt, Germany).
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