Formaldehyde

Manufactured by Maclin Biochemical Technology

Sourced in China

Formaldehyde is a colorless, flammable gas that is used in various industrial and laboratory applications. It has a pungent odor and is soluble in water, alcohol, and other organic solvents. Formaldehyde is a common fixative and preservative used in biological and medical laboratories.

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Formaldehyde solution (2 citations)

7 protocols using formaldehyde

Formaldehyde-based Pine Wood Veneer

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Formaldehyde (37%), urea (98%), sodium hydroxide, formic acid, and ammonium chloride used in this experiment were all analytical pure grade and purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (Shanghai, China). Nano-SiO₂ was purchased from Guangzhou Hongwu Material Technology Co., Ltd. (Guangzhou, China). All reagents were used directly without further purification. The veneer used was pine wood spin-cut veneer, width 400 mm × 400 mm, thickness 1.5 mm, made by the laboratory.

Xiao J., Guo D., Xia C., Li T, & Lian H. (2022). Application of Nano-SiO2 Reinforced Urea-Formaldehyde Resin and Molecular Dynamics Simulation Study. Materials, 15(24), 8716.

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Bioremediation of Petrochemical Wastewater

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Beer yeast sludge (containing Saccharomyces cerevisiae) was collected after the fermentation process in Wuchuan Yanjing brewery and further purification or modification, were finished in the laboratory. Real petrochemical wastewater samples were collected from the discharged sewage of Maoming Petrochemical Refining Co., Ltd (Guangdong, China). The other chemicals in this study were consumed as provided by suppliers without any further modifications. Formaldehyde, anhydrous ethanol, polyethylene glycol (molar mass: 600), K₂Cr₂O₇, 1,5-diphenyl carbazide, FeCl₃·6H₂O and FeCl₂·4H₂O were purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China). Hydrochloric acid, sulfuric acid, sodium hydroxide and ammonia water were provided by Tianjin Damao Chemical Reagent Co., Ltd. (Tianjin, China).

Long W., Chen Z., Shi J, & Yang S. (2022). Efficient Removal of Cr(VI) Ions in Petrochemical Wastewater Using Fe3O4@Saccharomyces cerevisiae Magnetic Nanocomposite. Nanomaterials, 12(18), 3250.

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ChIP Assay of FOXO3a Binding to TGF-β and HO-1 Promoters

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EZ-Magna Chromatin Immunoprecipitation Kits (Millipore) was applied for the ChIP assay according to the user’s manual. Briefly, 1×10⁷ human embryonic kidney 293 T (HEK-293T) cells were cross-linked in 1% formaldehyde (Macklin, Shanghai, China). The cell lysates were broken by sonication to produce DNA fragments of 200~1000 bp using the Bioruptor (Diagenode). Then normal rabbit IgG (Cell Signaling Technology, 2729) and anti-FOXO3a (Thermo Fisher Scientific, 720128) were used for immunoprecipitation reaction. After reverse cross-linking and DNA purification, the enriched DNA was subjected to q-PCR analysis using SYBR Green (Thermo Fisher Scientific). qPCR products were identified by agarose gel electrophoresis (AGE). The primers amplified genomic fragments containing putative FOXO3a sites on the TGF-β or HO-1 promoter, which were predicted by JASPAR database (http://jaspar.genereg.net/). Meanwhile, the promoter activity of TGF-β and HO-1 was predicted in BDGP: Neural Network Promoter Prediction (https://www.fruitfly.org/seq_tools/promoter.html/) and Promoter 2.0 Prediction (http://www.cbs.dtu.dk/services/Promoter/). The primer sequences used in qPCR are shown in Table S3.

Xu S., Zhang X., Ma Y., Chen Y., Xie H., Yu L., Wang J., Xu S, & Pan F. (2022). FOXO3a Alleviates the Inflammation and Oxidative Stress via Regulating TGF-β and HO-1 in Ankylosing Spondylitis. Frontiers in Immunology, 13, 935534.

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Synthesis of Novel Compounds

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All chemicals, including ZnCl₂ (Aladdin), phenol (Aladdin), formaldehyde (Macklin), melamine (Macklin), SeS₂ (Macklin), N-methy1-2-pyrrolidone (Aladdin), and CuSO₄ (Macklin), were used without further purification.

Zhang J., Zhang X., Xu C., Liu Y., Xu J., Miao Z., Yu H., Yan L., Zhang L, & Shu J. (2023). Dual synergistic effects assisting Cu-SeS2 electrochemistry for energy storage. Proceedings of the National Academy of Sciences of the United States of America, 120(13), e2220792120.

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Peach-Based Gelatin Production Protocol

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Gelatin (200 bloom, 12.5%)
was purchased from Hongcai Technology Development Co., Ltd. (Henan,
China). Acetic acid, formaldehyde, sodium hydroxide, sodium, and sulfate
anhydrous were purchase from Macklin Biochemical Co. Ltd. (Shanghai,
China). Citral essential oil was supplied by Ji’an Guoguang
spice factory (Ji’an, Jiangxi, China). Peaches (Amygdalus
persica) were picked at the season when they mature from
the planting base (102°.80’E, 24°.89’N, Kunming
city, Yunnan, China) in September 2021 (Chenggong District, Kunming,
Yunnan, China)

Li Z., Zheng M., He P., Gong W., Liu Z., Xu C, & Tai Z. (2022). Citral Essential Oil-Loaded Microcapsules by Simple Coacervation and Its Application on Peach Preservation. ACS Omega, 7(46), 42181-42190.

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Ginsenoside Re Extraction and Characterization

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Ginsenoside Re (Re purity) were purchased from Nanjing Chunqiu Biological Engineering Co., Ltd., and identified according to the Chinese Pharmacopoeia (2015 Edition). Perchloric acid, glacial acetic acid, anhydrous calcium chloride, and formaldehyde were obtained from Shanghai Macklin Biochemical Co., Ltd. (China). Leu-enkephalin was obtained from Sigma-Aldrich company (USA). Acetonitrile was obtained from Thermo Fisher Scientific company (USA). HPLC-grade formic acid and methanol were obtained from Dikma Technologies company (CA). The assay kits used to determine IL-2, IL-6, IL-12, and IgM were purchased from Jiancheng Bioengineering Institute (Nanjing, China).

Yu C., Fu J., Guo L., Yu M, & Yu D. (2022). Integrating Metabolomics and Network Pharmacology to Explore the Protective Effect of Ginsenoside Re against Radiotherapy Injury in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 5436979.

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Efficient Demulsification of Heavy Oil Emulsion

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Tetraethylene
pentaamine was purchased from
Beijing Tianyu Kanghong Chemical Technology Co., Ltd. P-Trifluoromethyl phenol was purchased from Shanghai Sahn Chemical
Technology Co., Ltd. Formaldehyde was purchased from Shanghai Macklin
Biochemical Technology Co., Ltd. Xylene and toluene were ordered from
Shanghai Jizhi Biochemical Technology Co., Ltd. Potassium hydroxide
was purchased from Shanghai Sibaiquan Chemical Co., Ltd. Potassium
hydroxide was purchased from Shanghai Si Bai Quan Chemical Co., Ltd.
Ethylene oxide (EO) and propylene oxide (PO) were purchased from Shandong
Zixiang Sales Chemical Co., Ltd. The tested oil sample was fluid produced
from a heavy oil block in Liaohe Oilfield. The physiochemical characteristics
are shown in Table 2.
Compared with the raw materials of
conventional demulsifier, the p-trifluoromethyl phenol
material used is more expensive
and cannot be applied in large quantities in the whole oil field production
process. However, it can achieve efficient demulsification performance
for the special heavy oil emulsion in a certain block, which is significantly
better than the various demulsifiers used in a certain block of Liaohe
Oilfield so as to solve the problem of demulsification and dehydration. P-Trifluoromethyl phenol is not a dangerous or control product,
and hence easily available. It can meet the needs of medium- and small-scale
production.

Wei L., Zhang L., Guo S., Jia X., Zhang Y., Sun C, & Dai X. (2021). Synthesis and Study of a New Type of Fluorinated Polyether Demulsifier for Heavy Oil Emulsion Demulsification. ACS Omega, 6(39), 25518-25528.

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