Log In Sign up
The largest database of trusted experimental protocols

Delta aminolevulinic acid hydrochloride ala

Manufactured by Frontier Scientific
Visit Supplier

Delta-aminolevulinic acid hydrochloride (ALA) is a laboratory chemical compound. It is a colorless crystalline solid. ALA is used as a research reagent in various scientific applications.

Automatically generated - may contain errors

Lab products found in correlation

Rhodamine 123, by Thermo Fisher Scientific (1 mentions) Pheophorbide a pha, by Frontier Scientific (1 mentions) Live dead cell viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) Protoporphyrin 9 ppix, by Frontier Scientific (1 mentions) Deferoxamine dfo, by Merck Group (1 mentions) Phenylmethanesulfonyl fluoride, by Merck Group (1 mentions) Pepstatin a, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Leupeptin, by Merck Group (1 mentions) Pheophorbide a, by Frontier Scientific (1 mentions)

4 protocols using delta aminolevulinic acid hydrochloride ala

1

Photodynamic Therapy Reagent Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Delta-aminolevulinic acid hydrochloride (ALA) from Frontier Scientific Inc. (Logan, UT) and rhodamine 123 from Life Technologies (Grand Island, NY) were dissolved in phosphate buffered saline (PBS) solution. Pheophorbide a (Pha) from Frontier Scientific Inc. and ABCG2 inhibitor Ko143 from Santa Cruz Biotechnology (Santa Cruz, CA) were dissolved in dimethyl sulfoxide (DMSO). All chemicals were sterilized through filters and stored in a −20 °C freezer.
Palasuberniam P., Yang X., Kraus D., Jones P., Myers K.A, & Chen B. (2015). ABCG2 transporter inhibitor restores the sensitivity of triple negative breast cancer cells to aminolevulinic acid-mediated photodynamic therapy. Scientific Reports, 5, 13298.
+ Open protocol
+ Expand
2

Optimizing PpIX Delivery for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Delta-aminolevulinic acid hydrochloride (ALA), purchased from Frontier Scientific Inc. (Logan, UT), was dissolved in phosphate buffered saline (PBS) solution. Protoporphyrin IX (PpIX) from Frontier Scientific Inc. was dissolved in DMSO solution. The solution of chemicals was sterilized by passing through filters and stored in a −20°C freezer. They were directly added into cell culture medium for treatment. The final concentration of DMSO in the medium was 0.1% or less. Live/Dead cell viability/cytotoxicity kit was purchased from Thermo Fisher Scientific and used according to manufacturer's instruction.
Yang X., Palasuberniam P., Myers K.A., Wang C, & Chen B. (2016). Her2 oncogene transformation enhances 5-aminolevulinic acid-mediated protoporphyrin IX production and photodynamic therapy response. Oncotarget, 7(36), 57798-57810.
+ Open protocol
+ Expand
3

Preparation of ALA and DFO Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Delta-aminolevulinic acid hydrochloride (ALA) from Frontier Scientific Inc. (Logan, UT) was dissolved in phosphate buffered saline (PBS) solution. Deferoxamine (DFO) from Sigma (St. Louis, MO) was dissolved in DMSO. All chemicals were sterilized through 0.22-µm filters and stored in a −20°C freezer.
Palasuberniam P., Kraus D., Mansi M., Braun A., Howley R., Myers K.A, & Chen B. (2019). Ferrochelatase Deficiency Abrogated the Enhancement of Aminolevulinic Acid-mediated Protoporphyrin IX by Iron Chelator Deferoxamine. Photochemistry and photobiology, 95(4), 1052-1059.
+ Open protocol
+ Expand
4

Fluorescent Compound Preparation and Handling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Delta-aminolevulinic acid hydrochloride (ALA) from Frontier Scientific Inc. (Logan, UT) was dissolved in phosphate-buffered saline (PBS) solution. PpIX and Pheophorbide A (Pha) were obtained from Frontier Scientific Inc. and dissolved in DMSO. Ko143 and palmitic acid were purchased from Sigma (St. Louis, MO) and dissolved in DMSO. Ethylenediaminetetraacetic acid (EDTA), zinc acetate, sodium dodecyl sulfate (SDS) and Triton X-100 were purchased from Sigma and dissolved in deionized water. Protease inhibitor cocktail (1× working solution) includes aprotinin (2 μg/mL), leupeptin (2 μg/mL), pepstatin A (1 μg/mL) and phenylmethanesulfonyl fluoride (PMSF, 1 mM), all from Sigma. All chemicals were sterilized through filtration using 0.22-μm pore size filters and stored in a −20°C freezer.
Howley R., Mansi M., Shinde J., Restrepo J, & Chen B. (2020). Evaluation of aminolevulinic acid-mediated protoporphyrin IX fluorescence and enhancement by ABCG2 inhibitors in renal cell carcinoma cells. Journal of photochemistry and photobiology. B, Biology, 211, 112017.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!