EPA ethyl ester and DHA ethyl ester (95% grade) were provided by Mochida Pharmaceutical (Tokyo, Japan). Standard laboratory chow (composed of 60% carbohydrate, 13% fat, and 27% protein on a caloric basis) were purchased from Oriental Yeast (Tokyo, Japan). An AHF diet was prepared by modification of a high-fat–high-sucrose diet with 1.25% cholesterol and 0.5% cholate (Oriental Yeast, Tokyo, Japan) [18 (link)]. The dietary component of AHF and fatty acid composition of each diet are presented in S1 and S2 Tables, respectively.
Standard laboratory chow
Manufactured by Oriental Yeast
Sourced in Japan
Standard laboratory chow is a nutritionally balanced feed formulated for laboratory animals. It provides the necessary nutrients to support the health and well-being of animals used in research and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using standard laboratory chow
1
Dietary Intervention with Fatty Acids
2
Zucker Diabetic Fatty Rat Model
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Five-week-old male Zucker Diabetic Fatty (ZDF) lean (?/+) and ZDF fa/fa rats were purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). ZDF fa/fa rats develop T2DM, while ZDF lean (?/+) rats represent ZDF +/+ or fa/+ rats and normal blood glucose level, and then these are used as control rats not to distinguish between ZDF +/+ and ZDF fa/+ rats. Wistar rats, for the preparation of phospholipid-fused mitochondria, were purchased from SLC Japan, Inc. (Tokyo, Japan). Animals were maintained on standard laboratory chow (Oriental Yeast Co., Tokyo, Japan) and water ad libitum, under a reverse dark-light cycle. Animals were fasted for > 18 hr before all experiments. All animals were treated humanely in accordance with guidelines issued by the National Institutes of Health (Bethesda, MD, USA), and all procedures were approved by the Animal Care Committee of Chiba University.
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