EP for all in vitro experiments was performed using an ECM 830 (BTX Technologies, Holliston, MA) square wave generator. The effect of EP was assessed at three energy settings (low: 500, intermediate: 1000, or high: 1500 V/cm) without varying the other pulse parameters (8 pulses, 100 μs pulse length, delivered at 1 Hz). Pulse delivery was performed using two stainless steel pin electrodes (25 mm length, 0.8 mm diameter, McMaster Carr, NJ) with 4 mm (for tumorspheres and transwell insert experiments) or 10 mm (for experiments in 24-well plates) spacing between electrodes, or using cuvettes (Micropulser Cuvette, BioRad, Hercules, CA) with a 4 mm electrode gap.
Micropulser cuvette
Manufactured by Bio-Rad
The Micropulser Cuvette is a laboratory equipment designed for electroporation, a technique used to introduce foreign molecules into cells. The cuvette provides a contained environment for the sample and electrodes, facilitating the application of an electric pulse to the cells.
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Lab products found in correlation
2 protocols using micropulser cuvette
1
Electroporation Protocol for Cell Cultures
2
Optimized Bacterial Electroporation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli MG1655 and K. pneumoniae KP22ΔRamA strains were prepared for electroporation. Cells grew to exponential phases in LB medium were washed twice and resuspended in 50 µL of sterile ddH2O and mixed with vectors. Electroporation was performed in a 1 mm Micropulser cuvette (Bio‐Rad) at 1800 V. Cells were recovered in LB medium at 37 °C for 1 h before plating onto selective LB agar plates and incubated for overnight at 37 °C.
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E. coli MG1655 and K. pneumoniae KP22ΔRamA strains were prepared for electroporation. Cells grew to exponential phases in LB medium were washed twice and resuspended in 50 µL of sterile ddH2O and mixed with vectors. Electroporation was performed in a 1 mm Micropulser cuvette (Bio‐Rad) at 1800 V. Cells were recovered in LB medium at 37 °C for 1 h before plating onto selective LB agar plates and incubated for overnight at 37 °C.
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