Anti cd151

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-CD151 is a laboratory reagent used for the detection and analysis of CD151, a cell surface protein that is involved in cell adhesion and signaling. This antibody can be used in various immunological techniques, such as flow cytometry, immunoprecipitation, and Western blotting, to study the expression and function of CD151 in different cell types and experimental systems.

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Lab products found in correlation

Anti integrin β1, by Cell Signaling Technology (2 mentions) Anti integrin α3, by Cell Signaling Technology (2 mentions) Subcellular protein fractionation kit, by Thermo Fisher Scientific (2 mentions) Axio imager m2 imaging system, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Anti pan cadherin, by Abcam (1 mentions) Quantitative western blot system, by LI COR (1 mentions) Irdye infrared dye, by LI COR (1 mentions) Tris glycine gels, by LI COR (1 mentions) Anti olig2, by Santa Cruz Biotechnology (1 mentions) Anti pakt s473, by Cell Signaling Technology (1 mentions) Anti flag, by Santa Cruz Biotechnology (1 mentions) Anti sox2, by Santa Cruz Biotechnology (1 mentions) Anti total akt, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Radioimmunoprecipitation assay buffer, by Merck Group (1 mentions) Anti p egfr, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Dab kit, by BD (1 mentions)

5 protocols using anti cd151

Immunostaining of Neurosphere Cells

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Neurosphere cells were collected by cytospin onto glass slides and fixed with 4% paraformaldehyde. Cells were permeabilized by Triton X-100 and immunostained with anti-CD151 (1:50; Santa Cruz Biotechnology, Dallas, TX, www.scbt.com), anti-integrin α3 (1:50), anti-integrin α6 (1:50), and anti-integrin β1 (1:100; Cell Signaling) antibodies following the protocol from Cell Signaling. Secondary antibodies were conjugated with Alexa488 or Cy3 (1:250). Immunofluorescent images were taken and analyzed using the ZEISS AxioImager M2 Imaging System with Axiovision software (Zeiss, Thornwood, NY, www.zeiss.com).

Tilghman J., Schiapparelli P., Lal B., Ying M., Quinones-Hinojosa A., Xia S, & Laterra J. (2016). Regulation of Glioblastoma Tumor-Propagating Cells by the Integrin Partner Tetraspanin CD151. Neoplasia (New York, N.Y.), 18(3), 185-198.

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Protein Extraction and Analysis

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Total cellular protein was extracted with radioimmunoprecipitation assay buffer (Sigma-Aldrich) containing protease and phosphatase inhibitors (Calbiochem, Billerica, MA, www.calbiochem.com). The Subcellular Protein Fractionation Kit was used for membrane protein extraction (Thermo Scientific). SDS-PAGE was performed with 50 μg of total proteins using 4% to 12% gradient Tris-glycine gels (LI-COR Biosciences, Lincoln, NE, www.licor.com). Western blot analysis was performed using the Quantitative Western Blot System, with secondary antibodies labeled by IRDye infrared dyes (LI-COR Biosciences). The primary antibodies were anti-CD151, anti-Olig2, anti-FLAG (Santa Cruz), anti-Sox2, anti-S⁴⁷³-pAkt, anti-total-Akt, anti-integrin α3, anti-integrin α6, anti-integrin β1 (Cell Signaling), anti-pan Cadherin (Abcam, Cambridge, MA, www.abcam.com), and anti-β-actin (Sigma-Aldrich).

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Immunofluorescence Staining of Cell Markers

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Cultured cells were fixed with 4 % paraformaldehyde for 15 min at room temperature, permeablized with triton (0.1 % in TBS) for 30 min and blocked with 5 % BSA in PBS for 1 h at room temperature. Cells were then incubated overnight at 4℃ with anti-CD151 (Santa Cruz, sc218216), anti-ITGα3 (Abcam, Ab242196), anti-p-EGFR (Abcam, ab40815). The corresponding secondary antibodies tagged with Cy3 and FITC were used (1:500, Beyotime Biotechnology). Finally, the samples were incubated in DAPI for 10 min (Life Technologies) for nuclear counterstain. Images were acquired using a Leica SP8 confocal microscope with optimal setting for the fluorescent markers used.

Zhu J., Cai T., Zhou J., Du W., Zeng Y., Liu T., Fu Y., Li Y., Qian Q., Yang X.H., Li Q., Huang J.A, & Liu Z. (2021). CD151 drives cancer progression depending on integrin α3β1 through EGFR signaling in non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research : CR, 40, 192.

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Immunohistochemical Analysis of CD151 and ADAM15

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Immunohistochemical assay was performed as previously described [15 ]. Adjacent sections of serial paraffin sections were incubated with anti-CD151 (Santa Cruz, CA, USA, 1:60 dilution in 5% BSA in PBS) and anti-ADAM15 antibodies (Abcam, London, UK, 1:200 dilution in 5% BSA in phosphate-buffered saline (PBS)) at 4 °C overnight and then incubated with the corresponding biotinylated secondary antibodies. The reactions were developed using the DAB Kit (BD Biosciences, San Jose, CA, USA). Detailed protocol was described in the Supplementary materials.

Zhou J., Wang A., Cai T., Li Y., Du W., Zhang Y., Zhang R., Zhang W., Zhu J., Zeng Y., Huang J.A, & Liu Z. (2022). Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression. Cell Death & Disease, 13(5), 486.

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Porcine PMEC PRRSV Infection and Receptor Detection

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Porcine PMECs (4th–5th passage) were seeded into 24-well plates for immunofluorescence. To detect viral antigens, cells were incubated for 12 hr and then
infected with the PRRSV strain HN. Immunofluorescence staining for PRRSV GP5 and N proteins was carried out at 48 hpi using polyclonal antibodies (Cat #bs-4504R
and Cat #23941R, Bioss, Beijing, China). To detect the receptors of PRRSV, the PMECs were fixed with 4% paraformaldehyde after a 24-hr incubation, and a routine
indirect immunofluorescence method was performed. Anti-CD151 (Cat #sc-18753-R, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CD163 (Cat #MCA2311GA, AbD
Serotec, Kidlington, Oxford, UK), and anti-CD169 (Cat #MCA2316GA, AbD Serotec) antibodies and goat anti-mouse (Cat #bs-0296G-FITC, Bioss) and goat anti-rabbit
(Cat #bs-0296G-FITC, Bioss) FITC-conjugated secondary antibodies were used.

LI P., YANG Z., MA S., HU G., DONG H, & ZHANG T. (2020). Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus. The Journal of Veterinary Medical Science, 82(9), 1404-1409.

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