Ccr10

Manufactured by R&D Systems

Sourced in United States

CCR10 is a lab equipment product manufactured by R&D Systems. It is designed for use in research and scientific applications. The core function of CCR10 is to facilitate the detection and measurement of specific cellular components or analytes.

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Lab products found in correlation

Cxcr6, by R&D Systems (2 mentions) Cd138, by BD (1 mentions) Cxcr5, by R&D Systems (1 mentions) Cpg odn 2006, by InvivoGen (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions) Taci fc, by R&D Systems (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Gapdh, by Merck Group (1 mentions) Facscanto 2, by BD (1 mentions) Hla dr, by BD (1 mentions) Cxcr1, by R&D Systems (1 mentions) Cx3cr1 sa011f11, by BioLegend (1 mentions) Facsaria 3, by BD (1 mentions) Cxcr4, by R&D Systems (1 mentions) Facscanto, by BD (1 mentions)

3 protocols using ccr10

Comprehensive Immune Cell Profiling Protocol

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Antibodies specific for the following molecules were used for flow cytometric analysis and cell sorting; CD3, CD4, CD8, CD19, CD20, CD27, CD38, CD40, CD86, CD138 and HLA-DR were from BD BioSciences. CCR6, CCR7, CCR9, CCR10, CXCR5 and α4β7 were from R&D Systems. αCD40 (clone 12E12, in-house), αBlimp-1 (Novus biological), αPax5 (eBioscience), αimmunoglobulins (Southern Biotech), αAPRIL (BioLegend) and αBAFF (R&D Systems) were used. The Live/Dead Fixable dead cell stain kit (Invitrogen) and the CellTrace CFSE Cell Proliferation kit (Invitrogen) were used. Cytofix/wash buffer kit (BD) was used for an intracellular Pax5 and Blimp-1 staining. Cells were acquired on an LSRII^™ or FACSCanto II^™ (BD Biosciences), and analysis was performed using FlowJo software (Tree Star Inc). Antibodies specific for pSTAT3 (Y705), phospho-p38, phospho-ERK, phospho-IκBα, STAT3, p38, ERK and IκBα were from Cell Signaling Technology. GAPDH was from Sigma-Aldrich. ox-LDL was from BTI Biomedical Technology. CCL19, CCL25, CCL27 and CCL28 were from eBioscience. TACI-Fc, BCMA-Fc and αBAFF mAb were from R&D Systems. CpG ODN2006 was from Invivogen. Poly ICLC was provided by Dr. Andres M. Salazar (Oncovir, Inc.)

Joo H., Li D., Dullaers M., Kim T.W., Duluc D., Upchurch K., Xue Y., Zurawski S., Le Grand R., Liu Y.J., Kuroda M., Zurawski G, & Oh S. (2014). C-Type Lectin-Like Receptor LOX-1 Promotes Dendritic Cell-Mediated Class-Switched B Cell Responses. Immunity, 41(4), 592-604.

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Multimarker Flow Cytometry Analysis

Cited 1 time

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The preparation of single‐cell suspensions was described previously (Fu et al., 2020 (link)). Antibodies specific for the following surface markers were used: Ly6G (1A8‐Ly6g), CD11b (M1/70), CD45 (30‐F11), CD205 (205yekta), and TLR2 (CB225) were purchased from eBioscience (San Diego, CA, USA); CCR3 (J073E5), CCR4 (2G12), CCR5 (HM‐CCR5), CCR6 (29‐2L17), CCR7 (4B12), CCR9 (CW‐1.2), CXCR2 (SA203G11), CXCR3 (CXCR3‐173), CXCR4 (L276F12), CXCR5 (L138D7), CXCR7 (8F11‐M16), and CX3CR1(SA011F11) were purchased from Biolegend (San Diego, CA, USA); CCR1 (R&D), CCR2 (R&D), CCR8 (1055C, R&D), CCR10 (R&D), CXCR1 (R&D), and CXCR6 (R&D) were purchased from R&D Systems (Minneapolis, MN, USA). All antibodies were used at the fold dilution described on the manufacturer's recommendation. Unstained cells, single stain, fluorescence minus one control, and isotype controls were used for setting laser voltages and for compensation. FCM data were obtained by FACSAria III (BD Biosciences, San Jose, CA, USA) and analyzed with FlowJo v. 10 (BD Biosciences, San Jose, CA, USA).

Fu C., Lu Y., Zhang Y., Yu M., Ma S, & Lyu S. (2021). Intermittent fasting suppressed splenic CD205+ G‐MDSC accumulation in a murine breast cancer model by attenuating cell trafficking and inducing apoptosis. Food Science & Nutrition, 9(10), 5517-5526.

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Single-cell surface marker profiling

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The expression of surface markers on single cells was determined with a FACS CANTO (BD Biosciences) using fluorochrome-conjugated monoclonal antibodies. The antibodies included CD3 (Sanquin), CD4 (Pharmingen), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCR10, CXCR4 and CXCR6 (all from R&D Systems).

Bunders M.J., van Hamme J.L., Jansen M.H., Boer K., Kootstra N.A, & Kuijpers T.W. (2014). Fetal exposure to HIV-1 alters chemokine receptor expression by CD4+T cells and increases susceptibility to HIV-1. Scientific Reports, 4, 6690.

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