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Naproxen

Manufactured by Cayman Chemical
Sourced in United States

Naproxen is a chemical compound used as a reference standard in analytical laboratory settings. It is a non-steroidal anti-inflammatory drug (NSAID) that can be used to verify the identity and purity of related pharmaceutical products. Naproxen is provided as a solid material in various formats to support analytical testing and quality control procedures.

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3 protocols using naproxen

1

Anti-inflammatory Compound Screening in Macrophages

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BMDMs were treated with with 0.125 – 0.5 mM indomethacin (Sigma-Aldrich), 0.25 – 1 mM (S)-ibuprofen (Cayman Chemical), 0.125 – 0.5 mM ketoprofen (Sigma-Aldrich), 0.25 – 1 mM Etodolac (Cayman Chemical), or 0.25 – 1 mM naproxen (Cayman Chemical), 0.25–1 mM rofecoxib, 5 mM sulforaphane (Cayman Chemical), 100 μM dimethylfumarate. NSAIDs were diluted in 1% DMSO. Cells were pretreated with NSAID for 1 hour prior to stimulation with 10 ng/mL LPS (L2880, Sigma-Aldrich). LDH assay performed according to manufacturer’s instructions (601170, Cayman Chemical). Reactive oxygen species (ROS) measured using H2DCFDA (D399, ThermoFisher) as described previously (Kavian et al., 2018 (link)). Sodium arsenite (Sigma-Aldrich), naphthalenedione (DMNQ, Cayman Chemical) and phenazine methosulfate (PMS, Cayman Chemical) at the indicated doses were used to induce ROS in BMDM.
MDMs were treated with indicated concentrations of indomethacin (Sigma-Aldrich), ketoprofen (Cayman Chemical), or sulforaphane (Cayman Chemical) for indicated durations.
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2

Caco-2 Cell Viability Assay for C. difficile

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Caco-2 cells were plated in black 96-well plates at 3 × 104 cells per well and incubated for 48 hours. After 48 hours, cell culture medium was refreshed, and cells were treated with indomethacin, ibuprofen (Cayman), naproxen (Cayman), aspirin, R2PPA, valeroyl silyciate (Cayman), celecoxib (Cayman), PGE2 (Cayman), or vehicle control [dimethyl sulfoxide (DMSO)] for 16 hours overnight. The next day, cells were intoxicated with filter-sterilized C. difficile VPI10463 cultures at a final dilution of 1:5 or 1:15 or mock-infected (BHI) and incubated for 8 hours. Cell viability was indicated by measuring ATP levels using the CellTiter Glo Reagent (Promega). Data were normalized to ATP levels of vehicle-treated and mock-infected cells.
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3

Naproxen and Venetoclax Dissolution Study

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Naproxen was supplied by Cayman Chemical (Ann Arbor, MI, USA), and venetoclax was obtained from AbbVie Inc. (Chicago, IL, USA). Vinylpyrrolidone-vinyl acetate copolymer (PVPVA64, Kollidon® VA 64) was purchased from BASF SE (Ludwigshafen, Germany). Methanol, water (LS-MS Grade), trifluoroacetic acid, and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). For the dissolution experiments, purified water without buffer was used. Unbuffered water was chosen because the modeling calculations in Section 2 were performed using water without any buffer system.
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