Mts reagent cell titer 96 aqueous one solution cell proliferation assay

Dulbecco’s Modified Eagle’s Medium (DMEM), ceftazidime, imipenem, and tobramycin were purchased from Sigma-Aldrich (Gillingham, UK), while the IDR-1018 was obtained from SYNTIDES (Shanghai, China). Cationic lipid 3β-[N-(N’, N’-dimethyl aminoethane)-carbamoyl] cholesterol hydrochloride (DC-Chol) and zwitterionic lipid 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were bought from Avanti-polar lipids (Birmingham, UK). Human lung carcinoma cell line (A549) was obtained from the American type culture collection (ATCC): ATCC number—CCL-185. The MTS reagent (cell titer 96^® aqueous one solution cell proliferation assay) was supplied by Promega (Southampton, UK). Distilled water was generated through Milli Q (Millipore Corporation, Bedford, MA, USA).

Synthesis of BPLPLs such as BPLPL-PLLA (1:100), BPLPL-PLGA50:50 (1:100), BPLPL-PLGA75:25 (1:100) was described previously (Xie et al., 2014 (link); Hu et al., 2016 (link)). The ratio of 1:100 represents the feeding molar ratio of BPLP either with lactic acid or a combination of lactic acid and glycolic acid. PLGA50:50 of molecular weight 55–65 kDa was purchased from Akina, Inc. (West Lafayette, IN). Other reagents including bovine serum albumin (BSA) and polyvinyl alcohol (PVA) of molecular weight 31–50 kDa were brought from Sigma-Aldrich (St. Louis, MO). MTS reagent (CellTiter 96®AQueous One Solution Cell Proliferation Assay) and Pierce BCA protein assay were obtained from Promega (Madison, WI) and ThermoFisher Scientific (Grand Island, NY), respectively. OxiSelect™ Intracellular Nitric Oxide (NO) Fluorometric Assay Kits were purchased from CellBioLabs, Inc. (San Diego, CA). Furthermore, human umbilical vein endothelial cells (HUVECs) was purchased from American Type Culture Collection (ATCC, Manassas, VA), while the culture media (Vasculife Basal Medium) and supplemental kits (Vasculife VEGF Lifefactors) were purchased from Lifeline Cell Technology (Frederick, MD). Other chemicals, if not specified were purchased from Sigma Aldrich.