The human pancreatic cancer cell lines PANC-1, SW1990, AsPC-1, BxPC-3 and CFPAC-1 were purchased from the Type Culture Collection Committee of the Chinese Academy of Sciences. Cells were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640), Dulbecco’s Modified Eagle Medium (DMEM), or Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 in a humidified incubator. Cobalt chloride (CoCl2)-treated cells were established as an in vitro hypoxia-mimetic condition. SW1990, BxPC-3, AsPC-1 and PANC-1cells were treated with 150 μM, 200 μM, 150 μM and 300 μM CoCl2 respectively for 24 h. To inhibit LRP1 function, pancreatic cancer cells were treated with the LRP1 antagonist receptor-associated protein (RAP) (0.5 μM) (human recombinant RAP, 553506 Sigma−Aldrich) for 3 days.
Sw1990
Manufactured by Merck Group
The SW1990 is a laboratory equipment product manufactured by Merck Group. It is designed to perform specific functions within a controlled laboratory environment. The core function of the SW1990 is to provide a precise and reliable tool for researchers and scientists to utilize in their work. No further details on the intended use or interpretation of the product's capabilities are provided.
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Lab products found in correlation
Bxpc 3, by Merck Group (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Panc 1, by Merck Group (4 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (4 mentions)
Sw1990, by American Type Culture Collection (4 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Aspc 1, by Merck Group (3 mentions)
Mia paca 2, by Merck Group (3 mentions)
Bxpc 3, by American Type Culture Collection (3 mentions)
Panc 1, by American Type Culture Collection (3 mentions)
Aspc 1, by American Type Culture Collection (3 mentions)
Cfpac 1, by Merck Group (2 mentions)
Amphotericin b, by Thermo Fisher Scientific (2 mentions)
Mia paca 2, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Psn 1, by American Type Culture Collection (1 mentions)
Pf 3758309, by Selleck Chemicals (1 mentions)
Kpt9274, by Selleck Chemicals (1 mentions)
10 protocols using sw1990
1
Hypoxia-Induced Pancreatic Cancer Cell Lines
2
Pancreatic Cancer Cell Line Cultures
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SW-1990, PSN-1, BxPC3 and PANC-1 cell lines were purchased from ATCC. MIA PaCa-2 and KP4 were purchased from Sigma-Aldrich and Accegen, respectively. MIA PaCa-2, PANC-1, SW-1990 were cultured in DMEM (Sigma-Aldrich) whereas PSN-1, BxPC3 and KP4 were cultured in RPMI1640 (Sigma-Aldrich). Both the Medias were supplemented with 10% Fetal Bovine Serum (Gibco) and 5% Penicillin-Streptomycin (Sigma-Aldrich). Cell lines were maintained at 37 °C with 5% CO2 in a cell culture incubator. Gemcitabine Hydrochloride was procured from Sigma Aldrich (catalogue number: G6423). PAK4 inhibitors (KPT-9274, catalogue number: S8444 and PF-3758309, catalogue number: S7094) were procured from Selleckchem.
3
Culturing Human Pancreatic Cell Lines
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Human pancreatic ductal cell line (HPNE) and PC cell lines, including PANC-1, SW1990,
HS766T, and CFPAC-1, were purchased from BeNa Culture Collection (Beijing, China) and
cultured in incubator with 100% humidity and 5% CO2 at 37°C. The HPNE cells
were grown in Dulbecco modified Eagle medium (DMEM; Sigma, St Louis, Missouri) containing
1 volume of M3 Base F culture medium (InCell Corp, San Antonio, Texas), 3 volumes of
glucose-free DMEM, 10% fetal bovine serum (FBS; (Invitrogen, Carlsbad, California), 5.5 mM
glucose, 10 ng/mL epidermal growth factor (EGF), and 50 µg/mL gentamycin. The DMEM medium
with 10% FBS was applied to culture PANC-1 and HS766T cells. SW1990 cells were maintained
in Leibovitz L-15 Medium (Sigma). Furthermore, CFPAC-1 cells were cultured in Iscove
Modified Dulbecco Medium (Sigma) adding 10% FBS.
HS766T, and CFPAC-1, were purchased from BeNa Culture Collection (Beijing, China) and
cultured in incubator with 100% humidity and 5% CO2 at 37°C. The HPNE cells
were grown in Dulbecco modified Eagle medium (DMEM; Sigma, St Louis, Missouri) containing
1 volume of M3 Base F culture medium (InCell Corp, San Antonio, Texas), 3 volumes of
glucose-free DMEM, 10% fetal bovine serum (FBS; (Invitrogen, Carlsbad, California), 5.5 mM
glucose, 10 ng/mL epidermal growth factor (EGF), and 50 µg/mL gentamycin. The DMEM medium
with 10% FBS was applied to culture PANC-1 and HS766T cells. SW1990 cells were maintained
in Leibovitz L-15 Medium (Sigma). Furthermore, CFPAC-1 cells were cultured in Iscove
Modified Dulbecco Medium (Sigma) adding 10% FBS.
4
Cell Line Culture for Pancreatic Cancer
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The human pancreatic adenocarcinoma cell lines, BxPC-3, AsPC-1 and SW1990, and the immortalized human endothelial cell line, EA.hy 926, were purchased from the American Type Culture Collection. The BxPC-3 and AsPC-1 cell lines were maintained in RPMI-1640 medium, while the SW1990 and EA.hy 926 cell lines were maintained in DMEM (both from Sigma-Aldrich; Merck KGaA). Each medium was replenished with 10% fetal bovine serum (FBS), 10 mg/ml streptomycin, 10,000 U/ml penicillin, and 25 µg amphotericin B (all from Gibco; Thermo Fisher Scientific, Inc.). All the cell lines were cultured at 37°C in a humidified incubator with 5% CO2.
5
Comprehensive PDAC Cell Line Cultivation
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Human PDAC cell lines (PANC1, MIA‐PaCa2, BxPC3, AsPC1, Capan‐2, SW1990, CFPAC1 and Suit2) were purchased from the ATCC. MIA‐PaCa2‐luc cells were purchased from JCRB Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan). MIA‐PaCa2, MIA‐PaCa2‐luc, BxPC3, AsPC1, Capan‐2, CFPAC1 and Suit2 cells were cultured in DMEM (Sigma‐Aldrich) supplemented with 10% FBS (Thermo Fisher Scientific). SW1990 and PANC1 cells were cultured in RPMI1640 medium (Sigma‐Aldrich) supplemented with 10% FBS.
6
Culturing Pancreatic Adenocarcinoma Cell Lines
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The three human pancreatic adenocarcinoma cell lines MIA PaCa-2 (cat. no. CRL-1420), SW 1990 (cat. no. CRL-2172) and BxPC-3 (cat. no. CRL-1687) and the immortalized human endothelial cell line EA.hy926 (cat. no. CRL-2922) were purchased from the American Type Culture Collection. MIA PaCa-2 and SW-1990 cells were cultured in DMEM and BxPC-3 cells were cultured in RPMI medium (both from Sigma Aldrich; Merck KGaA) in a 37°C humidified incubator with 5% CO2. These media were supplemented with 10% FBS, 10,000 U/ml penicillin, 25 µg/ml amphotericin B and 10 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc.).
7
Pancreatic Cancer Cell Line Characterization
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Cell lines were obtained from the American Type Culture Collection (AsPC-1, Panc10.05, SW-1990, MIA PaCa-2, PANC-1, HPAC, and HPAF-II) or were a gift from J. Fleming at MD Anderson Cancer Center (Pa01C, Pa02C, Pa14C, and Pa16C). Cells were maintained in either Dulbecco's minimum essential medium (Gibco; 11995-065; MIA PaCa-2, PANC-1, HPAC, HPAF-II, Pa01C, Pa02C, Pa14C, and Pa16C) or RPMI1640 (Gibco; AsPC-1, Panc 10.05, and SW-1990) supplemented with 10% fetal bovine serum (Sigma–Aldrich) as well as penicillin and streptomycin (Sigma–Aldrich). All cell lines were short-tandem repeat profiled to confirm their identity. All cell lines tested negative for mycoplasma contamination.
8
Establishment of Gemcitabine-Resistant Pancreatic Cancer Cell Lines
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Parent cell lines SW1990, Panc-1, and BxPC-3 were purchased from Procell (Wuhan, China). The HPDE6-C7 cell line and Capan-2 were maintained in our lab. GEM-resistant SW1990 and Panc-1 cell lines were established in our laboratory. Briefly, SW1990 and Panc-1 cell lines were exposed to a series of concentrations of gemcitabine (purity >98%; 5, 10, 20, 40, 80, 160, and 320 μM, Sigma-Aldrich) biweekly until cells became resistant to 320 μM of gemcitabine. BxPC-3 cell line was cultured in RPMI-1640 medium containing 10% FBS. SW1990, Panc-1, and the respective GEM-resistant cell lines were cultured in the DMEM medium containing 10% FBS, 100 U/mL of penicillin, and 100 mg/mL of streptomycin at 5% CO2, 37 °C.
9
Culturing Pancreatic and Endothelial Cells
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The PC cell lines PANC-1 and SW1990, immortalized human pancreatic ductal epithelial cells (HPDCs) and human lymphatic tube endothelial cells (HDLECs) were purchased from Guangzhou Genio Biotech Co., Ltd. The SW1990 and HPDC cells were cultured in DMEM (MilliporeSigma), the PANC-1 cells were cultured in RPMI-1640 (HyClone; Cytiva) and the HDLECs were cultured in Endothelial Cell Medium (ScienCell Research Laboratories, Inc.), each supplemented with 10% FBS (Thermo Fisher Scientific, Inc.) and 1% antibiotics (penicillin-streptomycin; Thermo Fisher Scientific, Inc.). Culture was performed under normoxic or hypoxic conditions in a humidified incubator at 37°C. The normoxic conditions were 20% O2, 5% CO2 and 75% N2, and the hypoxic conditions were 1% O2, 5% CO2 and 94% N2. Cells in the logarithmic growth stage were selected for subsequent experiments.
10
Culturing Pancreatic Cell Lines
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The human pancreatic ductal epithelial cell line HPDE6c7 (cat. no. BNCC359453) was obtained from BeNa Culture Collection. The PC cell lines BxPC-3 (cat. no. CL-0042), SW1990 (cat. no. CL-0448B) and PANC-1 (cat. no. CL-0184) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. HPDE6c7 and BxPC-3 cells were cultured in RPMI-1640 medium, while SW1990 and PANC-1 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS; MilliporeSigma) and 1% penicillin/streptomycin solution (Life Technologies; Thermo Fisher Scientific, Inc.). All cells were cultured at 37˚C in a humidified incubator with 5% CO2.
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