Synthetic 1,2-dioleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3- phospho-L-serine (POPS), 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt, DGS-NTA-Ni) and N-(lissamine rhodamine B sulfonyl)-1,2-dipalmitoylsn-glycero-3 phosphoethanolamine (Rhod-PE) were purchased from Avanti Polar Lipids. SNAP-cell 505 was obtained from New England Biolabs. Tetramethylrhodamine-5-maleimide was from Life Technologies. Lck inhibitor (7-Cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine) and rapamycin were obtained from Sigma-Aldrich. The antibodies used in this study were: mouse anti-pY142-CD3ζ (anti-CD247, 558489, 1:1000, BD PhosFlow), anti-pY418-Src (pY394-Lck, 560094, BD PhosFlow 1:500), anti-pY505-Lck (558552, BD PhosFlow, 1:3,000), anti-GAPDH (MAB374, Millipore, 1:50,000), rabbit anti-Lck (2984S, Cell signaling, 1:1,000), rabbit anti-pY416-Src (2101S, Cell signaling, 1:1,000). Validation of each antibody is provided on the manufacturer's websites.
Lck inhibitor 7 cyclopentyl 5 4 phenoxyphenyl 7h pyrrolo 2 3 d pyrimidin 4 ylamine
Manufactured by Merck Group
Sourced in United States
The Lck inhibitor (7-Cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine) is a chemical compound that functions as a selective inhibitor of the Lck (Lymphocyte-specific protein tyrosine kinase) enzyme. Lck is a critical signaling molecule involved in T-cell activation and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Merck Group (2 mentions)
Rapamycin, by Merck Group (2 mentions)
Tetramethylrhodamine 5 maleimide, by Thermo Fisher Scientific (2 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions)
Snap cell 505, by New England Biolabs (2 mentions)
Synthetic 1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
N lissamine rhodamine b sulfonyl 1 2 dipalmitoylsn glycero 3 phosphoethanolamine rhod pe, by Avanti Polar Lipids (2 mentions)
Anti py505 lck, by BD (2 mentions)
Horseradish peroxidase conjugated anti rabbit igg antibody, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase conjugated anti mouse igg antibody, by Santa Cruz Biotechnology (1 mentions)
Tfiib, by Santa Cruz Biotechnology (1 mentions)
Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions)
Dokdo mark protein size marker, by Elpis Biotech (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Biotin protein ligase bira kit, by Avidity (1 mentions)
4 protocols using lck inhibitor 7 cyclopentyl 5 4 phenoxyphenyl 7h pyrrolo 2 3 d pyrimidin 4 ylamine
1
Reconstituting Lipid Bilayers for T-cell Signaling
2
Leptins and Signaling Pathway Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Lck, p-Lck (Tyr394), STAT3, p65, p-p65 (Ser536), LepR, GAPDH, and TFIIB were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The antibody against p-STAT3 (Tyr705) was obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-rabbit IgG-horseradish peroxidase-conjugated antibody and anti-mouse IgG-horseradish peroxidase-conjugated antibody were obtained from Santa Cruz Biotechnology. Polyvinylidene difluoride (PVDF) membranes were obtained from Millipore Corporation (Bedford, MA, USA). Dokdo-MARKTM protein size marker was obtained from ElpisBiotech (Daejeon, Korea). Rat and murine recombinant leptins were purchased from PeproTech (Rocky Hill, NJ, USA). Lck inhibitor (7-cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo [2,3-d]pyrimidin-4-ylamine) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The highest available grade was selected for all materials.
3
Reconstituting Lipid Bilayers for T-cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synthetic 1,2-dioleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3- phospho-L-serine (POPS), 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt, DGS-NTA-Ni) and N-(lissamine rhodamine B sulfonyl)-1,2-dipalmitoylsn-glycero-3 phosphoethanolamine (Rhod-PE) were purchased from Avanti Polar Lipids. SNAP-cell 505 was obtained from New England Biolabs. Tetramethylrhodamine-5-maleimide was from Life Technologies. Lck inhibitor (7-Cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine) and rapamycin were obtained from Sigma-Aldrich. The antibodies used in this study were: mouse anti-pY142-CD3ζ (anti-CD247, 558489, 1:1000, BD PhosFlow), anti-pY418-Src (pY394-Lck, 560094, BD PhosFlow 1:500), anti-pY505-Lck (558552, BD PhosFlow, 1:3,000), anti-GAPDH (MAB374, Millipore, 1:50,000), rabbit anti-Lck (2984S, Cell signaling, 1:1,000), rabbit anti-pY416-Src (2101S, Cell signaling, 1:1,000). Validation of each antibody is provided on the manufacturer's websites.
4
Recombinant mPD-L1 and ICAM-1 Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
His6-tagged mPD-L1 with BirA sequence were produced in CHO cells (ATCC) as described previously76 (link). Biotinylation was performed in vitro using the BirA biotin-protein ligase kit (Avidity). Recombinant mouse ICAM-1 with a human IgG1 Fc tag was from Biolegend. Wide type and α3A2 mutant of gp33:H2-Db were made by the National Institutes of Health Tetramer Core Facility at Emory University.
PE-conjugated anti-mPD-1 (clone J43, 1:20), anti-mTCR Vα2 (clone B20.1, 1:20), anti-mCD8α (clone 53-6.7, 1:20), isotype RatIgG2a,λ (clone B39-4, 1:20), isotype RatIgG2a,κ (clone A95-1, 1:20), and isotype american hamster IgG2,κ (clone B81-3, 1:20) were from BD Biosciences. PE-conjugated anti-mPD-L1 (clone MIH5, 1:20) and biotinylated anti-human IgG1 (clone HP6070, 1:200) were from ThermoFisher Scientific. BV421-conjugated anti-CD4 (clone GK1.5, 1:100), PE-conjugated anti-mCD8α (clone 53-6.7, 1:100), anti-H2-Db (clone KH95, 1:20), and isotype ratIgG1,κ (clone RTK2071, 1:20) were from Biolegend. APC-conjugated anti-CD44 (clone IM7, 1:20) was from Tonbo biosciences. Biotinylated anti-His tag (1:40) was from Qiagen.
SHP1 and SHP2 inhibitor NSC87877 was from Santa Cruz. Lck inhibitor 7-Cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3‑d]pyrimidin-4-ylamine was from Sigma Aldrich.
PE-conjugated anti-mPD-1 (clone J43, 1:20), anti-mTCR Vα2 (clone B20.1, 1:20), anti-mCD8α (clone 53-6.7, 1:20), isotype RatIgG2a,λ (clone B39-4, 1:20), isotype RatIgG2a,κ (clone A95-1, 1:20), and isotype american hamster IgG2,κ (clone B81-3, 1:20) were from BD Biosciences. PE-conjugated anti-mPD-L1 (clone MIH5, 1:20) and biotinylated anti-human IgG1 (clone HP6070, 1:200) were from ThermoFisher Scientific. BV421-conjugated anti-CD4 (clone GK1.5, 1:100), PE-conjugated anti-mCD8α (clone 53-6.7, 1:100), anti-H2-Db (clone KH95, 1:20), and isotype ratIgG1,κ (clone RTK2071, 1:20) were from Biolegend. APC-conjugated anti-CD44 (clone IM7, 1:20) was from Tonbo biosciences. Biotinylated anti-His tag (1:40) was from Qiagen.
SHP1 and SHP2 inhibitor NSC87877 was from Santa Cruz. Lck inhibitor 7-Cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3‑d]pyrimidin-4-ylamine was from Sigma Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!