Rtca software pro 2.3.2

HT-29 (10⁴ cells/well), HCT-116 (10³ cells/well), HepG2 (10⁴ cells/well), and Hep3B (5 × 10³ cells/well) were seeded in 96-well plates (E-Plate 96, Roche Applied Science, Mannheim, Germany). Real-time dynamic cell proliferation was monitored in 30 min intervals during 106 h using the xCELLigence system (Agilent Technologies Inc., Santa Clara, CA, USA). Cell index values were calculated using the RTCA Software Pro (2.3.2.) (Agilent Technologies Inc.). All curves were normalized at the beginning of the treatment period (10 h after seeding) applying the RTCA software [54 (link)].

Human cancer cell lines: PC3, RPMI-7951, MDA-MB-231, FaDu, or mouse cancer cell lines: TRAMPC2, RM1, B16F10, EMT6 or CT26 were cultured a day before to form a monolayer on 96-well electronic microplates. Cells were infected with CAL1 or SNV1 at different MOIs in the presence of 20% human sera or fetal bovine serum in a final volume of 200 µL/well. Cell attachment and proliferation from selected wells of the plate were monitored and recorded every 15 min using the RTCA MP. Cytolysis was analyzed in real-time or at 24-, 48-, 72-, and 96-h post-infection using RTCA software Pro 2.3.2 (Agilent, CA, USA). A parameter termed cell index was used to quantify cell attachment status based on detected cell-electrode impedance. Results reflect the average of 3 replicate wells.