Beta actin ac 15

Carbenoxolone (CBX), BAPTA-AM and thapsigargin (TG) were purchased from Tocris Bioscience (Ellisville, MO). APT102, the recombinant form of soluble CD39, was kindly provided by Dr. Ridong Chen at APT Therapeutics Inc. (St. Louis, MO). All other chemicals and cell culture media were from Sigma-Aldrich (St. Louis, MO) and other culture reagents from Life Technologies (Carlsbad, CA), unless otherwise stated.
Rabbit anti-mouse P2X7 antibody (#APR-004) was obtained from Alomone labs (Jerusalem, Israel); beta-actin (AC-15, #ab6276) from Abcam (Cambridge, MA); Flotillin-2 (B6, #sc-28320) from Santa Cruz Biotechnology, Inc. (Dallas, TX); Caveolin-1 (7C8, #NB100-615) from Novus Biologicals (Littleton, CO); phospho-AKT (S473) (#9271), phospho-PRAS40 (T246) (#2997), phospho-S6K (T389) (#9205), phospho-S6 (S235/236) (#2211) from Cell Signaling Technology (Danvers, MA); HRP-conjugated goat anti-mouse (#31438), donkey anti-rabbit (#31458) and mouse anti-goat IgG (#31400) and the SuperSignal West Femto Maximum Sensitivity Substrate reagents (#PI-34096) were from Thermo Scientific (Rockford, IL).

The following antibodies were used: beta-actin (AC-15) (ab6276; Abcam Inc., Toronto, ON, Canada); MLH1 (4C9C7) (3515; Cell Signaling Technology Inc., Danvers, MA); MLH3 (NBP1-00106; Novus Biologicals, Oakville, ON); ERCC8 (ABIN486848; Antibodies-online, Atlanta, GA); PAR (4335-MC-100; Trevigen®, Gaithersburg MD); PARP1 (H-250)(SC-7150, Santa Cruz Biotechnology Inc., Dallas, TX); MRE11A (4895, Cell Signaling Technology Inc.); RAD51 (14B4) (ab213; Abcam Inc.); phospho-histone γ-H2AX (Ser139) (JBW301) (05-636, Millipore, Temecula, CA); and Geminin (10802-1-AP, Proteintech, Rosemont, IL).