Annexin 5 conjugated with fluorescein isothiocyanate fitc

The following drugs and reagents used carrageenan (degree IV), human neutrophil myeloperoxidase, o-dianisidine dihydrochloride, trypan blue dye, oyster glycogen, and lipopolysaccharide were purchased from Sigma Chemical Co. (St Louis, MO, USA). Dexamethasone was purchased from Ache Pharmaceutical Laboratories S.A., São Paulo, SP, Brazil. The chemical reagents were purchased from Vetec, Rio de Janeiro, RJ, Brazil, and Reagen, Rio de Janeiro, RJ, Brazil. The enzyme-linked immunosorbent assay (ELISA: EIA) for the quantification of mouse interleukin 1β, interleukin 17A, and interleukin 10 was purchased from eBioscience, Inc., San Diego, CA, USA. The antibodies (Ly6G conjugated with phycoerythrin (PE), CD11b conjugated with PE-Cy™7, Annexin V conjugated with fluorescein isothiocyanate (FITC), and 7-AAD) used in the apoptosis/necrosis assay were purchased from BD Biosciences, San Jose, CA, USA. All other reagents used were of analytical grade and obtained from various commercial sources.