Sodium tetraborate (Na2B4O7.10H2O), HepesNa, Coomassie Brillant Blue R-250, N-[3-(2-Furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG), lisinopril, and Coomassie Brillant Blue G-250 were bought from Sigma-Aldrich. NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare Life Sciences. Captopril was purchased from Alfa Aesar.
Coomassie brillant blue r 250
Manufactured by Merck Group
Coomassie Brilliant Blue R-250 is a synthetic dye commonly used in laboratory procedures for the detection and quantification of proteins. It is a versatile staining agent that binds to proteins, allowing their visualization and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Lisinopril, by Merck Group (1 mentions)
Nhs activated sepharose 4 fast flow, by GE Healthcare (1 mentions)
Hepes na, by Merck Group (1 mentions)
Captopril, by Thermo Fisher Scientific (1 mentions)
Coomassie brillant blue g 250, by Merck Group (1 mentions)
Sodium tetraborate, by Merck Group (1 mentions)
Novex 10 zymogram plus gelatin gels, by Thermo Fisher Scientific (1 mentions)
2 protocols using coomassie brillant blue r 250
1
Affinity Purification of Angiotensin-Converting Enzyme
2
Gelatin Zymography for MMP-2 Activity
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MMP-2 activity was determined by PAGE zymography based on prior reports [36 (link)]. Cell lysates (10μg) were added to nonreducing sample buffer (1:3, v/v) and loaded on to Novex 10% zymogram plus (gelatin) gels (Thermo Fisher Scientific, Halethorpe, MD); electrophoresis was performed at 100 V for ∼2 h at 4 °C. The gels were then rinsed trice with 2.5% Triton X-100 to remove SDS and renature the MMPs in renaturing buffer. After a brief wash in distilled water, gels were incubated overnight at 37 °C in developing buffer with gentle agitation. The gels were stained with Coomassie® Brillant Blue R-250 (Sigma), and MMP activities were detected as transparent bands on the blue background.
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