Pastorex toxo kit

Serologic tests were performed at the Center for Diagnosis and Study of Parasitic Diseases, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania. All serum samples were tested using a Pastorex Toxo kit (Bio-Rad, Marnes-la-Coquette, France), which simultaneously detects IgG and/or IgM antibodies to T. gondii using latex particle agglutination. This test has shown an excellent ability to detect T. gondii antibodies in patients with acute and chronic toxoplasmosis [22 (link),26 ,27 (link)]. Testing, quality controls, and interpretation of results were based on the manufacturer’s criteria.

All serum samples were tested at the Center for Diagnosis and Study of Parasitic Diseases, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania. A Pastorex Toxo kit (Bio-Rad, Marnes-la-Coquette, France) was used to simultaneously detect the presence of IgG and/or IgM antibodies to T. gondii. Previous reports showed an excellent ability to detect T. gondii antibodies in patients with acute and chronic toxoplasmosis for this latex particle agglutination test [29 ,30 (link),31 (link)].
To identify the presence of serum anti-T. gondii IgM antibodies and evaluation of IgG avidity, the enzyme-linked fluorescent assay (ELFA) designed for VIDAS (bioMérieux, Marcy-l’Etoile, France) was used. ELFA IgM (VIDAS Toxo IgM kit) has a sensitivity of 100% and a specificity of 98.6% [32 (link),33 ]. An accuracy of 93.4% in detecting a T. gondii infection dating more than 4 months was recently found for IgG avidity Vidas [34 (link)]. Quality controls and testing and interpretation of results were based on the manufacturer’s criteria.

Serum samples were tested at the Center for Diagnosis and Study of Parasitic Diseases, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania. Pastorex Toxo kit (Bio-Rad, Marnes-la-Coquette, France), a latex particle agglutination assay, was used for simultaneously detection of immunoglobulin G (IgG) and/or immunoglobulin M (IgM) antibodies to T. gondii. Pastorex Toxo demonstrated an excellent ability to detect T. gondii antibodies in patients with acute and chronic toxoplasmosis [17 ,18 (link),20 (link)]. Testing, quality controls and interpretation of results were based on manufacturer’s criteria.