Rabbit anti nucleolin

Manufactured by Santa Cruz Biotechnology

Rabbit anti-Nucleolin is a primary antibody produced in rabbits that specifically binds to the nucleolin protein. Nucleolin is a multifunctional protein involved in various cellular processes, including ribosome biogenesis, chromatin remodeling, and cell proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti myc, by Santa Cruz Biotechnology (2 mentions) Mouse anti brdu antibody, by Merck Group (2 mentions) A1 confocal microscope, by Nikon (2 mentions) Nis elements software, by Nikon (2 mentions) Anti ubf, by Santa Cruz Biotechnology (1 mentions) Anti ha, by Fortrea (1 mentions) Volocity 3d image analysis software, by PerkinElmer (1 mentions) Rabbit anti h3, by Abcam (1 mentions) Metamorph microscopy automation image analysis software, by Molecular Devices (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Mouse anti p54nrb, by BD (1 mentions) Mouse anti coilin, by Merck Group (1 mentions) Ix70 deltavision rt deconvolution system microscope, by Olympus (1 mentions)

4 protocols using rabbit anti nucleolin

Immunofluorescence Staining of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells plated on glass coverslips were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS; 20 min, 25 ºC), permeabilized with 0.1% TX-100 in PBS (10 min, 25 ºC) and incubated (1 h, 25 ºC) with the following primary antibodies diluted in PBS with 4% fetal bovine serum: mouse or rabbit anti-HA (Covance 901503 or 902302, respectively); mouse or rabbit anti-UBF (Santa Cruz sc-13125 or sc-9131, respectively); mouse anti-myc (Santa Cruz sc-40); or rabbit anti-Nucleolin (Santa Cruz sc-13057). For FuRD labeling, cells were incubated with FuRD (2 mm, 15 min, 37ºC)^{41 (link)} before fixing and staining with mouse anti-BrdU antibody (Sigma, B8434), as described above. After incubating with primary antibodies, the cells were incubated with fluoroscein isothiocyanate- or tetramethylrhodamine-conjugated anti-mouse or anti-rabbit secondary antibody (1 h, 25 ºC), mounted in mounting media (1 mg/ml p-phenylenediamene in 1:9 PBS:glycerol), and imaged using a Nikon A1 confocal microscope and Nikon NIS elements software (Nikon, Melville, NY, USA).

Gonyo P., Bergom C., Brandt A.C., Tsaih S.W., Sun Y., Bigley T.M., Lorimer E.L., Terhune S.S., Rui H., Flister M.J., Long R.M, & Williams C.L. (2017). SmgGDS is a transient nucleolar protein that protects cells from nucleolar stress and promotes the cell cycle by regulating DREAM complex gene expression. Oncogene, 36(50), 6873-6883.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells plated on glass coverslips were fixed in 4% paraformaldehyde in PBS (20 min, 25ºC), permeabilized with 0.1% TX-100 in PBS (10 min, 25ºC), and incubated (1 h, 25ºC) with the following primary antibodies diluted in PBS with 4% FBS: mouse or rabbit anti-HA (Covance 901503 or 902302, respectively), mouse or rabbit anti-UBF (Santa Cruz sc-13125 or sc-9131, respectively), mouse anti-myc (Santa Cruz sc-40), or rabbit anti-Nucleolin (Santa Cruz sc-13057). For FuRD labeling, cells were incubated with FuRD (2 mM, 15 min, 37ºC)^{41 (link)} before fixing and staining with mouse anti-BrdU antibody (Sigma, B8434), as described above. After incubating with primary antibodies, the cells were incubated with FITC- or TRITC-conjugated anti-mouse or anti-rabbit secondary antibody (1 h, 25ºC), mounted in mounting media (1 mg/ml p-phenylenediamene in 1:9 PBS:glycerol), and imaged using a Nikon A1 confocal microscope and Nikon NIS elements software.

+ Open protocol

+ Expand

Immunofluorescence Microscopy of Histone Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed in 2 % paraformaldehyde (Electron Microscopy Sciences) for 10 min at room temperature (RT) and permeabilized in 0.5 % Triton X-100 (BioShop) for 5 min at RT. The primary antibodies used were rabbit anti-H3.3 (Abcam), rabbit anti-H3K9me3 (gift from P Singh), mouse anti-H3K9me3 and mouse anti-H4K20me3 (H Kimura), mouse anti-B23 (Santa Cruz Biotechnology), rabbit anti-nucleolin (Santa Cruz Biotechnology), and mouse anti-FLAG (Sigma-Aldrich). The secondary antibodies used were rabbit or mouse Cy2, Cy3, and Cy5 (Jackson ImmunoResearch Laboratories). Cells were soaked in DAPI and mounted in buffered glycerol with 4 % n-propyl gallate.
Images were collected on an Olympus IX81 inverted microscope equipped with a Cascade II CCD camera (Photometrics) using either a 60× or 100× oil-immersion objective lenses. MetaMorph Microscopy Automation & Image Analysis Software (Molecular Devices) was used to collect images. Images were processed with Volocity 3D Image Analysis Software (PerkinElmer) and Photoshop (Adobe). Graphs and statistics were constructed using GraphPad Prism (GraphPad Software Inc) and reported as standard error of the mean. Line scan data was collected using ImageJ (National Institute of Health) and the histograms constructed using GraphPad Prism.

Rapkin L.M., Ahmed K., Dulev S., Li R., Kimura H., Ishov A.M, & Bazett-Jones D.P. (2015). The histone chaperone DAXX maintains the structural organization of heterochromatin domains. Epigenetics & Chromatin, 8, 44.

+ Open protocol

+ Expand

RNA ISH for CCAT1 Isoform Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect CCAT1-L and CCAT1-S, RNA ISH was carried out as previously described with in vitro transcribed digoxigenin-labeled antisense probes^{32 (link)}. For colocalization studies, cells were co-stained with rabbit anti-Nucleolin (Santa Cruz Biotechnology), mouse anti-p54^nrb (BD) and mouse anti-Coilin (Sigma). The nuclei were counterstained with DAPI. Images were taken with a Zeiss LSM 510 microscope or with an Olympus IX70 DeltaVision RT Deconvolution System microscope.

Xiang J.F., Yin Q.F., Chen T., Zhang Y., Zhang X.O., Wu Z., Zhang S., Wang H.B., Ge J., Lu X., Yang L, & Chen L.L. (2014). Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell Research, 24(5), 513-531.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!