G9951

Manufactured by Promega

The G9951 is a luminometer designed for the detection and quantification of luminescent signals. It is a compact and versatile laboratory instrument that measures light output from luminescent reactions, such as those used in bioluminescence assays. The G9951 provides accurate and reproducible measurements of luminescent samples.

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Lab products found in correlation

White wall, by Thermo Fisher Scientific (1 mentions) G8092, by Promega (1 mentions) Polarstar omega, by BMG Labtech (1 mentions) Ab277086, by Abcam (1 mentions) L4391 1mg, by Merck Group (1 mentions) Dy201 05, by R&D Systems (1 mentions) Dy318 05, by R&D Systems (1 mentions) N7143 5mg, by Merck Group (1 mentions)

3 protocols using g9951

Evaluating Caspase Activities in Cells

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Caspase-1, caspase-3/7, and caspase-8 activities were determined by using a caspase-Glo 1 (G9951, Promega), caspase-Glo 3/7 (G8092, Promega), or caspase-8 assay kit (G8202, Promega) according to the manufacturer’s instructions. Cells were seeded in 96-well plate with white wall (Nunc). After treatment, an equal volume of caspase-Glo 1, caspase-Glo 3/7, or caspase-8 reagent was added to the cell culture medium and shaken for 30 min. Luminescent recording was performed with POLAR star Omega (BMG Labtech). For peritoneal lavage detection, 50 μl of assay reagent was added to peritoneal lavages of an equal volume isolated from mice by using 1 ml of PBS.

Zhang P., Liu Y., Hu L., Huang K., Hong M., Wang Y., Fan X., Ulevitch R.J, & Han J. (2021). NLRC4 inflammasome–dependent cell death occurs by a complementary series of three death pathways and determines lethality in mice. Science Advances, 7(43), eabi9471.

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Caspase Activity Quantification

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Caspase (G8090, G9951, Promega) activity were addressed in cells after 24 hours treatment or infection according to the manufacturer instructions.

Planès R., Pinilla M., Santoni K., Hessel A., Passemar C., Lay K., Paillette P., Valadão A.L., Robinson K.S., Bastard P., Lam N., Fadrique R., Rossi I., Pericat D., Bagayoko S., Leon-Icaza S.A., Rombouts Y., Perouzel E., Tiraby M., Zhang Q., Cicuta P., Jouanguy E., Neyrolles O., Bryant C.E., Floto A.R., Goujon C., Lei F.Z., Martin-Blondel G., Silva S., Casanova J.L., Cougoule C., Reversade B., Marcoux J., Ravet E, & Meunier E. (2022). Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. Molecular Cell, 82(13), 2385-2400.e9.

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NLRP3 Inflammasome Modulation by MSC-EVs

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NLRP3 inflammasome activity and downstream mediators of NLRP3 activation was evaluated in rat atrial fibroblasts, THP-1 macrophages, and atrial tissue lysates. Rat atrial fibroblasts and THP-1 macrophages were treated with BM-MSC, EDC or UC-MSC EVs before exposure to 500 ng/mL lipopolysaccharide (LPS, L4391-1MG, Sigma) and 20 µM nigericin (N7143-5MG, Sigma) to activate the NLRP3 inflammasome. A bioluminescent assay was used to evaluate secreted caspase-1 activity (G9951, Promega) while IL-1β and IL-18 in culture supernatants and tissue lysates were quantified using ELISA (IL-1β; DY201-05 & IL-18; DY318-05, R&D) and Luminex discovery assay (LXSARM-06, R&D) respectively. The specificity of caspase-1 activation was validated by including the caspase-1 inhibitor (YVAD-CHO, Promega). NLRP3 activity was evaluated in atrial tissue lysates using a commercial ELISA (ab277086, Abcam).

Parent S., Vaka R., St Amant J., Kahn S., Van Remortel S., Bi C., Courtman D., Stewart D.J, & Davis D.R. (2024). Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation. Theranostics, 14(2), 608-621.

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