A 785 nm laser diode (FPL785S-250, Thorlabs, Ely, UK) with maximum output of 250 mW and controlled via customized LabVIEW software using a controller (CLD1015, Thorlabs) was used in an optical tweezers assembly, as shown in Figure 1 . The beam is directed via a dichroic mirror (DM) into the back aperture of a Nikon ×100, 1.3 NA objective lens (Thorlabs). A microscope stage is mounted on a Three-Axis Motorized Translation Stage (MT3/M-Z8, Thor labs). The stage is controlled via customized LabVIEW software using DC Servo Motor Controllers (KDC101, Thor labs). The sample is illuminated from below by an LED (MCWHL5, LEDD1B, Thorlabs) and imaged by a CCD camera (DCC1545M, Thorlabs).
Kdc101
Manufactured by Thorlabs
Sourced in United States
The KDC101 is a kinetic mount designed for precise angular adjustment of optical components. It features a compact design with a clear aperture of 10 mm and supports a maximum load of 0.5 kg. The mount provides ±2° of angular adjustment in both the pitch and yaw axes and is equipped with a locking mechanism to secure the position.
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3 protocols using kdc101
1
Optical Tweezers Setup with Laser Diode
2
Femtosecond Pulsed Laser Setup
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Pulsed laser: The mode-locked monochromatic femtosecond pulsed laser we used in our experiments is emitted from a Ti:Sapphire crystal in an optical cavity (Spectra Physics, Tsunami). The laser has a center wavelength of 800.32 nm and a full-width-half-maximum length of ~ 10.5 nm. The laser power is in the range of 0.3 ~ 1.2 W with the pulse duration of ~ 200 fs and the repetition rate of 80.7 MHz. The laser beam is guided by a series of broadband dielectric mirrors and finally focused by a 10× (Edmund Optics) objective lens to achieve a Gaussian intensity profile with a 1/e 2 radius of 20 μm on the quartz surface. An optical shutter controlled by a digital controller (KDC101, Thorlabs) is used to turn on/off the laser (see Figure 5a).
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Motorized Rotation Stage for TRUS Transducer
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