Bioreagent grade buffer salts

Ethylene oxide, propylene oxide, 1,2-epoxybutane, potassium tert-butoxide, tetrahydrofuran, 18-crown-6 ether, methanol, naphthalene, potassium metal, α-cyano-4-hydroxycinnamic acid (CHCA), sodium trifluoroacetate (NaTFA), and diphenylhexatriene (DPH), bioreagent grade buffer salts (sodium chloride, potassium chloride, calcium chloride, magnesium chloride, and HEPES) and Triton X-100 were purchased from Sigma Aldrich and used without further purification. 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) was purchased from Avanti Polar Lipids as a 10 mg/mL solution in chloroform.

Pluronic P188 was donated by BASF (Wyandotte, MI). Dihydroxyl PEO homopolymers (M_n = 8,000 and 20,000 Da) were purchased from EMD Millipore (Billerica, MA). Dihydroxyl PEO (M_n = 7,800 Da) was purchased from Polymer Source (Dorval, Montreal) and used in the synthesis of the inverted PPO-PEO-PPO triblock copolymer. For the synthesis of methoxy-PEO-PPO-hydroxyl chain end functionalized diblocks (denoted Me-PEO-PPO-OH), poly(ethylene glycol) methyl ether (Me-PEO-OH) with M_n = 2,000 and 5,000 Da were purchased from Polymer Source (Dorval, Montreal) and EMD Millipore (Billerica, MA), respectively. Monomers (propylene oxide and ethylene oxide, purity >99.0%), potassium tert-butoxide, 18-crown-6 ether, potassium metal and naphthalene were purchased from Sigma-Aldrich. Bioreagent grade buffer salts (sodium chloride, potassium chloride, calcium chloride, magnesium chloride, and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)) and Triton X-100 were purchased from Sigma-Aldrich.