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2 protocols using pelargonin chloride

1

Comprehensive Analysis of Phenolic Compounds

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Sodium carbonate, Folin–Ciocalteu reagent, potassium chloride, sodium acetate trihydrate, aluminum chloride hexahydrate, sodium hydroxide, sodium nitrite, iron(III) chloride hexahydrate, 2,4,6-tris(2-pyridyl)-s-triazine, Trolox, hydrochloric acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), ethanol, and HPLC-grade solvents (water, acetonitrile, formic acid, and methanol) were purchased from Merck (Darmstadt, Germany). Phenolic compounds (pelargonin chloride, epigallocatechin, cyanidin chloride, malvidin chloride, petunidin 3-O-β-glucosidase chloride, catechin, cyanidin 3-O-glucosidase chloride, gallic acid, myricetin, caffeic acid, epigallocatechin gallate, quercetin, p-coumaric acid, and epicatechin) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Distilled water was used in the analyses, except for individual phenolic compound determination.
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2

Quantification of Polyphenol Standards in LC-MS/MS

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The following compounds were used as standards in LC-MS/MS analyses: fumaric acid (99%, Sigma-Aldrich), pyrogallol (98%, Sigma-Aldrich), rutin (94%, Sigma-Aldrich), chlorogenic acid (95%, Sigma-Aldrich), gallic acid (99%, Merck), syringic acid (95%, Sigma-Aldrich), t-ferulic acid (99%, Sigma-Aldrich), caffeic acid (98%, Sigma-Aldrich), pelargonin chloride (98%, Sigma-Aldrich), quercitrin (97%, Sigma-Aldrich), salicylic acid (99%, Sigma-Aldrich), p-coumaric acid (98%, Sigma-Aldrich), luteolin-7-O-glu (99%, AppliChem), rosmarinic acid (96%, Sigma-Aldrich), pyrogallol (98%, Sigma-Aldrich), apigenin (95%, Sigma-Aldrich), kaempferol (96%, Sigma-Aldrich), and isorhamnetin (98%, ExtraSynthese, Genay-France).
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