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2 protocols using anti mouse igg h l alexa fluor 488

1

Investigating Inflammatory Mediator Modulation

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TNF-α (Catalog #: ab6671), Collagen 10 (Catalog #: ab49945), MMP3 (Catalog #: ab52915), MMP13 (Catalog #: ab39012), iNOS (Catalog #: ab15323), UBA1 (Catalog #: ab34711) Adamts4 (Catalog #: ab1855722) and Adamts5 (Catalog #: ab41037) were purchased from Abcam (MA, USA), Adalimumab (Catalog #: A2010) and Glucosamine (Catalog #: S6400) were purchased from Selleckchem (TX, USA), UBE2N (Catalog #: 6999), phospho-RIP1 (Catalog #: 65746), NF-κB phospho-p65 (Catalog #: 3033), NF-κB p65 (Catalog #: 8242), RIP1 (Catalog #: 3943), Anti-rabbit IgG (H + L) Alexa Fluor 555 (Catalog #: 4413) and Anti-mouse IgG (H + L) Alexa Fluor 488 (Catalog #: 4408) were purchased from Cell Signaling Technology (MA, USA), Vimentin (Catalog #: SC6260), HA-Tag (Catalog #: SC7392), IκB (Catalog #: SC1643) and phospho-IκB (Catalog #: SC8404) were purchased from Santa Cruz Biotechnology (CA, USA), Spermidine (Catalog #: 05292) and Aggrecan (Catalog #: AB1031) were purchased from Sigma (MO, USA), CLYD (Catalog #: 1110-1-AP) and TRIM21 (Catalog #: 12108-1-AP) were purchased from PeproTech (NJ, USA).
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2

Comprehensive Protein Analysis Protocol

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Total protein was extracted using RIPA lysis buffer containing protease and phosphatase inhibitor cocktail, EDTA-free, 100X (APT006 or APT008, AntiProtech Inc.) Western blot analysis was performed using standard procedures as previously described [18 (link)]. The antibodies used were as follows: anti-NEK9 (cat. no. ab138488, Abcam), anti-ROBO1 (cat. no. 20219-1-AP, ProteinTech Group, Inc.), anti-Flag (cat. no. MA5558, AntiProtech Inc.), anti-STAT3 (cat. no. 12640, Cell Signaling Technology, Inc.), anti-p-STAT3 (cat. no. 9145, Cell Signaling Technology, Inc), anti-mouse IgG H&L (Alexa Fluor 488) (cat. no. ab150113, Abcam), anti-TRIM28 (cat. no. 15202-1-AP, ProteinTech Group, Inc.), anti-CTTN (cat. no. sc-55579, Santa Cruz Biotechnology, Inc.) anti-pCTTN (cat. no. 4569, Cell Signaling Technology, Inc.), anti-p100 (cat. no. 4882, Cell Signaling Technology, Inc.), anti-HA (cat. no. 51064-2-AP, ProteinTech Group, Inc.), anti-p-Ser (cat. no. sc-81514, Santa Cruz Biotechnology, Inc.) and anti-β-actin (cat. no. A2228, clone AC-74, Millipore Sigma).
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