Log In Sign up
The largest database of trusted experimental protocols

Anti α smooth muscle actin antibody 1a4

Manufactured by Merck Group
Visit Supplier

The Anti-α-smooth muscle actin antibody (1A4) is a lab equipment product that specifically binds to the alpha-smooth muscle actin protein. This antibody can be used in various research applications to detect and identify cells expressing this protein.

Automatically generated - may contain errors

Lab products found in correlation

Mec13, by BD (2 mentions)

2 protocols using anti α smooth muscle actin antibody 1a4

1

Embryonic Tissue Staining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
The embryos for immunohistochemical and whole-mount staining were fixed in 4% paraformaldehyde. Embryos for immunohistochemical staining were embedded in paraffin and cut into 6- to 7-μm thick sections. Tissues for frozen sections were embedded in OCT (Tissue-Tek) and cut into 8-μm thick sections. The frozen sections were fixed in acetone and acetone–chloroform. The haematoxylin and eosin staining procedure followed standard protocol. The immunohistochemical and immunofluorescent staining with anti-α-smooth muscle actin antibody (1A4, Sigma, 1:400 dilution) or anti-CD31 antibody (MEC13.3, BD, 1:400 dilution) followed basic immunoperoxidase procedures or immunostaining procedure for fluorophore-conjugated secondary antibodies. The procedures for whole-mount anti-CD31 staining were described previously4 (link).
Fisher O.S., Deng H., Liu D., Zhang Y., Wei R., Deng Y., Zhang F., Louvi A., Turk B.E., Boggon T.J, & Su B. (2015). Structure and vascular function of MEKK3–cerebral cavernous malformations 2 complex. Nature Communications, 6, 7937.
+ Open protocol
+ Expand
2

Immunohistochemical and Whole-Mount Staining of Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
The embryos for immunohistochemical and whole-mount staining were fixed in 4% paraformaldehyde. Embryos for immunohistochemical staining were embedded in paraffin and cut into 6- to 7-μm thick sections. Tissues for frozen sections were embedded in OCT (Tissue-Tek) and cut into 8-μm thick sections. The frozen sections were fixed in acetone and acetone-chloroform. The hematoxylin and eosin staining procedure followed standard protocol. The immunohistochemical and immunofluorescent staining with anti-α-smooth muscle actin antibody (1A4, Sigma, 1:400 dilution) or anti-CD31 antibody (MEC13.3, BD, 1:400 dilution) followed basic immunoperoxidase procedures or immunostaining procedure for fluorophore-conjugated secondary antibodies. The procedures for whole-mount anti-CD31 staining were described previously4 (link).
Fisher O.S., Deng H., Liu D., Zhang Y., Wei R., Deng Y., Zhang F., Louvi A., Turk B.E., Boggon T.J, & Su B. (2015). Structure and vascular function of MEKK3–cerebral cavernous malformations 2 complex. Nature Communications, 6, 7937.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!