in 50 mM Tris at pH 7.8, 500 mM KCl, and 10% glycerol. SAXS measurements
were made using a PANalytical X-ray generator with a Cu target (at
50 mA, 40 kV) and an Anton-Paar SAXSess system, which is an updated
model of the Kratky camera.40 The signal
was acquired with an electronic CMOS detector over a scattering angle
range (2θ) from 0.14 to 10°. Data processing was performed
with SAXSquant software from Anton-Paar and consisted of desmearing
to correct for the line slit geometry of the Kratky method.41