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Saxsess system

Manufactured by Anton Paar
Sourced in Austria

The SAXSess system is a small-angle X-ray scattering (SAXS) instrument designed for structural characterization of materials. It provides information about the size, shape, and internal structure of nanomaterials, polymers, and biological macromolecules.

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2 protocols using saxsess system

1

SAXS Characterization of SufU Proteins

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SaSufU and BsSufU were diluted to ∼25 mg/mL
in 50 mM Tris at pH 7.8, 500 mM KCl, and 10% glycerol. SAXS measurements
were made using a PANalytical X-ray generator with a Cu target (at
50 mA, 40 kV) and an Anton-Paar SAXSess system, which is an updated
model of the Kratky camera.40 The signal
was acquired with an electronic CMOS detector over a scattering angle
range (2θ) from 0.14 to 10°. Data processing was performed
with SAXSquant software from Anton-Paar and consisted of desmearing
to correct for the line slit geometry of the Kratky method.41
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2

SAXS Analysis of Hydrated Starch

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SAXS experiments were performed using an SAXSess system (Anton-Paar GmbH, Austria) equipped with a PW3830 X-ray generator (PANalytical B.V., Netherlands), operated at 50 mA and 40 kV, using Cu Kα radiation with a wavelength of 0.1542 nm as the X-ray source. The moisture content of freeze-dried starch samples were adjusted to 50% by adding a certain amount of water, and equilibrated at 20 °C for 24 h, before the detection. The sample was filled into a sample cell and measured for 10 min. The data, recorded using an image plate, were collected by the IP Reader software program with a PerkinElmer ® Storage Phosphor System. All data were normalized, and the background intensity and smeared intensity were removed using the SAXS Quant 3.0 software program for further analysis.
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