LILRB1 D3D4, antibody MDB1 and RIFIN genes were synthesized and subcloned (GenScript, NJ) into pVRC8400 vectors, with HRV3C cleavable His or Fc tag. DNAs of RIFIN with either LILRB1 or MDB1 were co-transfected into Expi293 Gnti- cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems). Six days post transfection, culture supernatants were harvested, and affinity purified with cOmplete™ His-Tag Purification Resin (Roche) or protein A resin (GE) by following manufacture’s protocols.
Expi293 gnti cells
Manufactured by Thermo Fisher Scientific
The Expi293 GnTi- cells are a genetically engineered cell line derived from human embryonic kidney (HEK) 293 cells. They are designed for efficient and high-yield transient protein expression. The cells feature a knockout of the GnT-I gene, which results in the production of proteins with homogeneous, non-fucosylated glycosylation patterns.
Automatically generated - may contain errors
3 protocols using expi293 gnti cells
1
LILRB1, MDB1, and RIFIN Protein Production
2
LAIR1 and RIFIN Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LAIR1 (wild type and mutants), antibodies (MGC34, MGD21, MGJ5, MGM1) and RIFIN genes were synthesized and subcloned (GenScript, NJ) into pVRC8400 vectors, with HRV3C cleavable His or Fc tag. DNAs were transfected into either Expi293F cells or Expi293 GnTI- cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems). Six days post transfection, culture supernatants were harvested, and affinity purified with cOmplete™ His-Tag Purification Resin (Roche) or protein A resin (GE) by following manufacture’s protocols.
3
LILRB1, MDB1, and RIFIN Protein Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LILRB1 D3D4, antibody MDB1 and RIFIN genes were synthesized and subcloned (GenScript, NJ) into pVRC8400 vectors, with HRV3C cleavable His or Fc tag. DNAs of RIFIN with either LILRB1 or MDB1 were co-transfected into Expi293 Gnti- cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems). Six days post transfection, culture supernatants were harvested, and affinity purified with cOmplete™ His-Tag Purification Resin (Roche) or protein A resin (GE) by following manufacture’s protocols.
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