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Ethyl acetate 1 2 13c2

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Ethyl acetate-1,2 13C2 is a stable isotope-labeled compound used as a research tool. It consists of a two-carbon ethyl acetate molecule with two carbon atoms replaced with the carbon-13 isotope. This product is typically used in scientific research applications that require the use of a specifically-labeled compound.

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Ethyl acetate (1318 citations) 13C2 acetate (2 citations)

2 protocols using ethyl acetate 1 2 13c2

1

Measuring de novo Palmitate Synthesis

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After 4 days of treatment, with vehicle or FASNi, cells were either washed twice with cold PBS and harvested for MS/MSALL, or incubated with 3 mM Ethyl acetate-1,2 13C2 (SIGMA ALDRICH, 283819) for 7 h (1 h for the time-lapse experiment) to measure de novo palmitate synthesis by GC/MS.
Bartolacci C., Andreani C., Vale G., Berto S., Melegari M., Crouch A.C., Baluya D.L., Kemble G., Hodges K., Starrett J., Politi K., Starnes S.L., Lorenzini D., Raso M.G., Solis Soto L.M., Behrens C., Kadara H., Gao B., Wistuba I.I., Minna J.D., McDonald J.G, & Scaglioni P.P. (2022). Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer. Nature Communications, 13, 4327.
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2

Lipidomic Analysis of de novo Palmitate Synthesis

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Lipidomic experiments. After 4 days of treatment, with vehicle or FASNi, cells were either washed twice with cold PBS and harvested for MS/MS ALL , or incubated with 3 mM Ethyl Acetate-1,2 13 C2 (SIGMA ALDRICH, 283819) for 7 hours (1 hour for the time-lapse experiment) to measure de novo palmitate synthesis by GC/MS. Fatty acid profiling by GC-MS. Total fatty acid profiles were generated by a modified GC-MS method previously described (Quehenberger et al., 2011) . The lipid extract was spiked with 100uL of 0.5µg/mL FA standard mixture (FA(16:0{ 2 H31}), FA(20:4 ω6{ 2 H8}) and FA(22:6 ω3{ 2 H5}) in methanol, then hydrolyzed in 1 mL of 0.5M potassium hydroxide solution prepared in methanol at 80 ºC for one hour. Hydrolyzed FA were extracted by adding 2mL of dichloromethane/water
Bartolacci C., Andreani C., Vale G., Berto S., Melegari M., Crouch A.C., Baluya D.L., Kemble G., Hodges K., Starrett J.H., Politi K., Starnes S.L., Lorenzini D., Raso M.G., Solis L.M., Behrens C., Kadara H., Gao B., Gerber D.E., Wistuba I.I., Minna J.D., McDonald J.G., & Scaglioni P.P. (2021). Targetingde novolipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer.
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