Sc 26643 r

Manufactured by Santa Cruz Biotechnology

Sc-26643-R is a laboratory product designed for research purposes. It is a solution that can be used for various applications in a laboratory setting. The core function of this product is to facilitate specific research activities, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ax10 microscope, by Zeiss (2 mentions) Sc 7557, by Santa Cruz Biotechnology (2 mentions) Anti phospho erk antibody, by Cell Signaling Technology (1 mentions) Abc kit for color development, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 26643 r

Immunohistochemical Analysis of Colon Tissues

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Colon tissues were fixed in 4% paraformaldehyde and embedded in paraffin. After incubation with blocking buffer, the sections were incubated with a goat polyclonal antivimentin antibody (sc-7557, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA), a rabbit polyclonal anti-F4/80 antibody (sc-26643-R, 1:50 dilution; Santa Cruz Biotechnology), or a rabbit polyclonal anti-phospho-ERK antibody (no. 4370, 1:50 dilution; Cell Signaling Technologies, Danvers, MA) overnight at 4°C. After washing, the sections were incubated with donkey anti-goat IgG or bovine anti-rabbit IgG, and the slides were stained with an ABC kit for color development (sc-2018; Santa Cruz Biotechnology). Immunohistochemistry was assisted by Translational Pathology Core Laboratory (TPCL) of UCLA. Images were analyzed with a Zeiss AX10 microscope at magnification of 200×.

Yoo J.H., Ho S., Tran D.H., Cheng M., Bakirtzi K., Kubota Y., Ichikawa R., Su B., Tran D.H., Hing T.C., Chang I., Shih D.Q., Issacson R.E., Gallo R.L., Fiocchi C., Pothoulakis C, & Koon H.W. (2014). Antifibrogenic Effects of the Antimicrobial Peptide Cathelicidin in Murine Colitis-Associated Fibrosis. Cellular and Molecular Gastroenterology and Hepatology, 1(1), 55-74.e1.

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Immunohistochemical Analysis of Colon Tissue

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Colon tissues were fixed in 4% paraformaldehyde and embedded in paraffin. After incubation with blocking buffer, sections were incubated with a goat polyclonal anti-vimentin antibody (sc-7557, Santa Cruz biotechnology, Santa Cruz, CA, 1:50 dilution), a rabbit polyclonal anti-F4/80 antibody (sc-26643-R, Santa Cruz, 1:50 dilution) or a rabbit polyclonal anti-phospho-ERK antibody (#4370, Cell signaling, Danvers, MA, 1:50 dilution) overnight at 4°C. After washing, sections were incubated with donkey anti-goat IgG or bovine anti-rabbit IgG and slides were stained with an ABC kit for color development (Santa Cruz, sc-2018). Immunohistochemistry was assisted by Translational Pathology Core Laboratory (TPCL) of UCLA. Images were analyzed with a Zeiss AX10 microscope at magnification of 200×.

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