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Tlr4 sirna

Manufactured by RiboBio
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Sourced in China

TLR4 siRNA is a laboratory reagent designed for gene silencing studies. It is a short, double-stranded RNA molecule that targets the TLR4 (Toll-like receptor 4) gene, which plays a role in the immune response. The primary function of TLR4 siRNA is to temporarily reduce the expression of the TLR4 gene in cells, allowing researchers to investigate the biological effects of TLR4 knockdown.

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Lab products found in correlation

Control sirna, by RiboBio (2 mentions) Lipofectamine rnaimax reagent kit, by Thermo Fisher Scientific (1 mentions)

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SiRNAs (265 citations)

2 protocols using tlr4 sirna

1

Knockdown of TLR4 in RAW264.7 cells

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TLR4 siRNA (CAATTCTGTTGCTTGTATA) and control siRNA (targeting β-actin) were synthesized by Guangzhou RiboBio Co., Ltd, China. RAW264.7 cells were cultured in a 24-well plate and siRNA (50 nM) was added by Lipofectamine® RNAiMAX Reagent Kit (Invitrogen, USA). After 24 h, the cells were incubated with Tris-HCl (pH 8.0) or Eh-rPrx (5 μg/mL).
Li X., Feng M., Zhao Y., Zhang Y., Zhou R., Zhou H., Pang Z., Tachibana H, & Cheng X. (2021). A Novel TLR4-Binding Domain of Peroxiredoxin From Entamoeba histolytica Triggers NLRP3 Inflammasome Activation in Macrophages. Frontiers in Immunology, 12, 758451.
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2

Silencing TLR4, TRIF, and MyD88 in RAW264.7 Cells

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TLR4 siRNA (target sequence: CAATTCTGTTGCTTGTATA), TRIF siRNA (target sequence: GGGAAGACCACACCTATAA), MyD88 siRNA (target sequence: GACTGATTCCTATTAAATA), and control siRNA (sequence unknown) were synthesized by Guangzhou RiboBio Co., LTD. RAW264.7 cells in the logarithmic growth phase were placed in a 6-well plate (2.5 × 105 cells/well), treated with siRNA (50 nM) for 24 h, and then incubated with or without recombinant proteins (5 μg/mL) for 24 h.
Li X., Zhang Y., Zhao Y., Qiao K., Feng M., Zhou H., Tachibana H, & Cheng X. (2020). Autophagy Activated by Peroxiredoxin of Entamoeba histolytica. Cells, 9(11), 2462.
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