Aconitase activity was determined by monitoring the conversion of isocitrate to cis-aconitate, which produces absorbance at a wavelength of 240 nm. The Aconitase Activity Microplate assay kit (ab109712, abcam, Shanghai, China) was employed per the manufacturer's recommended protocol [45 (link)].
Aconitase activity microplate assay kit
Manufactured by Abcam
Sourced in China
The Aconitase Activity Microplate assay kit is a laboratory tool used to measure the activity of the enzyme aconitase. Aconitase is a key enzyme in the citric acid cycle, a metabolic pathway that plays a crucial role in energy production within cells. This assay kit provides a quantitative method to assess aconitase activity in various sample types.
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Lab products found in correlation
2 protocols using aconitase activity microplate assay kit
1
Aconitase Activity Measurement Protocol
2
Mitochondrial Enzyme Activity Assay
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Mitochondria were isolated from cells as described above. Mitochondrial complex I, II, and IV activities were measured as described previously (Spinazzi et al, 2012 ). Aconitase activity was measured using Aconitase Activity Microplate Assay Kit (Abcam). Citrate synthase activity was measured from the same sample using Citrate Synthase Activity Kit (BioVision) and was used to normalize the mitochondrial complex activity.
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