Soluble Aβ42 oligomers or soluble scrambled Aβ42 oligomers were prepared as described (32 (link), 104 (link)). One milligram of lyophilized human Aβ42 (Anaspec) or scrambled Aβ42 (Anaspec) was dissolved in 1 ml of 1,1,1,3,3,3-hexafluoro-2-propanol (Fisher Scientific) to prevent aggregation, portioned into 10-μg aliquots, air dried, and stored at −80 °C. For use in experiments, an aliquot was thawed at room temperature and then dissolved in 100% dimethyl sulfoxide, then diluted into PBS to make a 100 μM solution. The solution was incubated for 16 h at 4 °C and then diluted to a final concentration for use in experiments. The following agonists were used in this study: 1 μM PNU-120596 (Alomone Labs), 2 μM RJR-2403 Oxalate (Alomone labs), 1 μM NS-3861 (Tocris Bioscience), and 1 μM Carbamoylcholine chloride (carbachol) (Tocris Bioscience). Aβcore (YEVHHQ) and inactive Aβcore (SEVAAQ) peptides were prepared as described (43 (link)).
Scrambled aβ42
Manufactured by AnaSpec
Scrambled Aβ42 is a lab equipment product from AnaSpec that serves as a control peptide for amyloid-beta (Aβ) studies. It is a synthetic peptide that has the same amino acid composition as Aβ42, but with a randomized sequence, preventing it from forming the characteristic amyloid fibrils.
Automatically generated - may contain errors
2 protocols using scrambled aβ42
1
Preparation and Application of Amyloid-beta Oligomers
2
Oligomeric Aβ42 Preparation and Agonist Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Soluble Aβ42 oligomers or soluble scrambled Aβ42 oligomers were prepared as previously described (32) . One mg of lyophilized human Ab42 (Anaspec) or scrambled Aβ42 (Anaspec) was dissolved in 1mL of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Fisher Scientific) to prevent aggregation, portioned into 10μg aliquots, air-dried and stored at -80°C. For use in experiments, an aliquot was thawed at room temperature, and then dissolved in 100% DMSO then diluted into PBS to make a 100μM solution. The solution was incubated for 16 hours at 4°C and then diluted to a final concentration for use in experiments. The following agonists were used in this study: 1μM PNU-120596 (Alomone labs), 2μM RJR-2403 Oxalate (Alomone labs), 1μM NS-3861 (Tocris Bioscience), and 1μM Carbamoylcholine chloride (carbachol) (Tocris Bioscience). Aβcore (YEVHHQ) and inactive Aβcore (SEVAAQ) peptides were prepared as described previously (43) .
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